接种量和装液量对少根根霉直接发酵生木薯粉生产富马酸的影响  

The effect of inoculum concentration and liquid volume on fumaric acid prodution from raw tapioca flour by Rhizopus arrhizus

作　　者：韩卫强[1] 王芳[1] 谭天伟[1] 邓利[1] 

机构地区：[1]北京化工大学生物加工过程北京市重点实验室,北京100029

出　　处：《北京化工大学学报（自然科学版）》2011年第5期95-99,共5页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家"973"计划(2007CB707804);国家自然科学基金(20876012);北京市自然基金(2071002)

摘　　要：利用少根根霉(Rhizopus arrhizus,RH-07-13)直接发酵生木薯粉生产富马酸。探讨了摇瓶发酵中,不同的接种量和装液量对生产富马酸的影响,检测了发酵培养过程中的富马酸、葡萄糖、乙醇的变化以及发酵结束后的菌体生物量。结果表明,最佳的接种量为20%(体积分数),最佳的装液量为25 mL/250 mL。在此条件下,发酵168 h,富马酸产量达49.88 g/L,生物量达43.8 g/L。The direct production of fumaric acid from raw tapioca flour by a strain of Rhizopus arrhizus RH-07-13 has been investigated. The effects of varying the liquid volume and inoculum concentration on fumaric acid produc- tion were investigated. Fumaric aeid, glucose and ethanol concentrations during fermentation and the biomass a- mount after fermentation were recorded. The maximum yield of fumaric acid of 49.88 g/L and biomass production of 43.8 g/L were obtained using the optimal inoeulum concentration of 20% and the optimal liquid volume of 25 mL/250 mL.

关 键 词：少根根霉 富马酸 生木薯粉 同步糖化 接种量 装液量 

分 类 号：TQ921[轻工技术与工程—发酵工程]