利用多孔陶瓷球固定α-淀粉酶的研究  

Immobilization of α-amylase with the porous ceramic spheres

作　　者：曾清平[1] 王超萍[1] 崔灏[1] 李敬龙[1] 

机构地区：[1]山东轻工业学院食品与生物工程学院,山东济南250353

出　　处：《中国酿造》2011年第4期151-154,共4页China Brewing

摘　　要：选取粉煤灰为原料,以适当比例添加玻璃粉,以炭黑为成孔剂,以羧甲基纤维素为粘结剂来制备多孔陶瓷球,并以此为载体固定化α-淀粉酶。对戊二醛浓度、固定化pH、温度这几个影响固定化酶酶活的关键因素展开研究,以酶活回收率为指标,分别进行单因素试验和正交试验,探索多孔陶瓷球固定化α-淀粉酶的最佳条件。结果显示,自制的陶瓷球,富含大量气孔,利于酶的固定化,在戊二醛浓度1.6%、固定化pH值为6.8、温度为10℃条件下,制备的固定化α-淀粉酶酶活回收率最高,达到84.2%。α-amylase was immobilized with the porous ceramic spheres using the fly ash as raw material with the appropriate proportion of glass powder,carbon soot as pore forming agent,carboxymethyl cellulose as binder.The several key factors,such as glutaraldehyde concentration,immobilized pH value,temperature,which affect the activity of the immobilize α-amylase,were studied.The results showed that self-made ceramic sphere is rich in a large number of pores,which helps the enzyme immobilization.With the conditions of glutaraldehyde concentration 1.6%,immobilized pH value 6.8,temperature 10℃,activity recovery of immobilized α-amylase was the highest rate,reached 84.00%.

关 键 词：多孔陶瓷 交联法 Α-淀粉酶 固定化 

分 类 号：Q814.2[生物学—生物工程]