酶解醇洗法制备棉籽浓缩蛋白  

Preparation of cottonseed protein concentrate by enzymolysis and alcohol extraction

作　　者：潘晶[1] 陈思思[1] 张晖[1] 郭晓娜[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《粮食与饲料工业》2011年第9期50-52,共3页Cereal & Feed Industry

摘　　要：以预榨浸提棉籽粕为原料,先选用戊聚糖复合酶、糖化酶等植物水解酶对棉籽粕进行作用,再进一步用乙醇溶液浸洗制备棉籽浓缩蛋白。结果表明:在添加糖化酶1 600 U/g、戊聚糖复合酶0.60 fbg/g、料液比1∶10和温度50℃条件下酶作用140 min后,再以料液比1∶7、体积分数70%乙醇溶液和温度60℃条件下醇洗2次,每次45 min,得到产品浓缩蛋白的蛋白质质量分数为64%,蛋白质收率为78%。The pre-pressed cottonseed meal as material was firstly hydrolyzed by pentosan compound enzyme and glucoamylase, and then washed by ethanol to preparate protein concentrate. The result illustrated as that： Under the condition of adding glueoamylase 1 600 U/g, pentosan enzyme 0.60 fbg/g, solvent/solid ratio of 1 ： 10, temperature 50~C, the cottonseed meal was hydrolyzed for 140 rain, and then washed by 70% （v/v） ethanol at 60~C dipped for 2 times（once for 45 rain）, with solvent/solid ratio of 1 ： 7. The pro tein content of cottonseed protein concentrate prepared was 64~, and the yield of protein was 78~.

关 键 词：棉籽粕 棉籽浓缩蛋白 酶解 醇洗 戊聚糖复合酶 糖化酶 制备 

分 类 号：S816.35[农业科学—饲料科学]