纤维蛋白溶解酶SPFE-I的溶栓活性及纤溶机理研究  

Study on thrombolysis activity and fibrinolytic mechanism of SPFE-I

作　　者：秦芸桦[1,2] 张涛[1] 江波[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品工业科技》2011年第10期412-413,417,共3页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(20976073);江南大学校内自主科研项目(JUSRP21107)

摘　　要：采用体外实验和SDS-PAGE电泳法对纤维蛋白溶解酶SPFE-I的溶栓活性、抗凝活性及纤溶机理进行了研究。体外实验证明,该酶终浓度为0.12U/mL时具有体外抗凝血作用,血块在1.5U/mL酶液中的溶解率为72%;采用SDS-PAGE电泳法研究发现,该酶可以水解纤维蛋白原的β、γ链,激活纤维蛋白溶酶原形成纤维蛋白溶解酶,并降解纤维蛋白溶解酶原激活剂抑制剂-1。Thrombolysis activity,anticoaguiation activity and fibrinolytic mechanism of SPFE-I were studied by vivo experiment and SDS-PAGE. As a result,the enzyme had the function of anticoagulation in 0.12U/mL, and dissolving rate of blood clots in 1.5U/mL was 72% ;β and y-chains of fibrinogen were cut off by SPFE-I, plasminogen could be activted to from plasmin and plasminogen activator inhibitor- I was also able to be degraded by it.

关 键 词：纤维蛋白溶解酶-I 溶栓 纤溶机理 

分 类 号：TS201.4[轻工技术与工程—食品科学]