产脂肪酶细菌的筛选及发酵培养条件的优化  

Screening for Lipase-producing Bacterial Strains and Optimization of the Fermentation Conditions

作　　者：洪鲲[1] 张豪[1] 乙引[1] 张习敏[1] 

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001

出　　处：《贵州农业科学》2011年第9期103-105,109,共4页Guizhou Agricultural Sciences

基　　金：贵州省科技攻关项目"生物酶法生产生物柴油"[黔科合NY(2006)3031];贵州省科技创新人才团队建设项目"贵州省特色动植物资源保护与可持续利用科技创新人才团队"[黔科合人才团队(2009)4007]

摘　　要：为给扩大脂肪酶的生产源提供基础资料,对富含油脂的67份土壤样品进行了产脂肪酶细菌的筛选及目标菌株发酵培养条件的优化试验。结果表明:筛选分离出15株脂肪酶产生菌,经过对其发酵液脂肪酶活力的比较,菌株D9-1发酵液脂肪酶活力最高。目标菌株D9-1产脂肪酶最佳培养条件:最适氮源为1%蛋白胨,最适碳源为1%橄榄油,表面活性剂为1%OP-10,最适发酵温度30℃,摇床转速为170 r/min,发酵培养时间为24 h。15 lipase-producing bacterial strains were isolated from 27 soil samples rich in oil and D9-1 was selected as the most potential lipase-producing strain through comparing the lipase activity in fermentation broths of these strains.The results showed that the optimum nitrogen source is peptone,optimum carbon source is 1% olive oil,optimum surfactant is 1% OP-10,optimum temperature is 30℃,and when the rotating speed was 170 r/min,the lipase activity in fermentation broth reached the highest after 24 h.

关 键 词：脂肪酶 细菌 筛选 发酵 培养条件 

分 类 号：S182[农业科学—农业基础科学] S154