Development of a vector and host system and characterization of replication of plasmid pSQIO in moderately halophilic Nocardiopsis  

作　　者：Ana Zeng Tao. Wang Haiyang Xia Shiyuan Peng Weihua Chen Chenglin Jiang Lihua Xu Li Zhong Meijuan Shen Zhongjun Qin 

机构地区：[1]Key laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200032, China [2]Institute of Microbiology, Yunnan University, Kunming 650091, China

出　　处：《Acta Biochimica et Biophysica Sinica》2011年第9期738-743,共6页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China （30770045）, National Basic Research Program （2011CBA00801）, and Chinese Academy of Sciences Project （KSCX2-EW-G-13）.

摘　　要：The genus of Nocardiopsis is a new source of antibiotics, chemicals, and enzymes. Here we reported the development of a vector and host system in moderately halophilic Nocardiopsis via an oriT-mediated conjugation. By screening about 80 Nocardiopsis strains, 6 of them harbored 8 plasmids （18-80 kb）. The complete nucleotide sequence of pSQ10 consisted of 18,219bp, with 71.9% G ＋ C content, encoding 17 open reading frames, 5 of them resembled those of Streptomyces plasmids. A rep locus （iteron within the gene） was identified for replication in Nocardiopsis sp. YIM 90083, and rep protein bound to its iteron sequence. This system may be useful for gene cloning and expression in Nocardiopsis.The genus of Nocardiopsis is a new source of antibiotics, chemicals, and enzymes. Here we reported the development of a vector and host system in moderately halophilic Nocardiopsis via an oriT-mediated conjugation. By screening about 80 Nocardiopsis strains, 6 of them harbored 8 plasmids （18-80 kb）. The complete nucleotide sequence of pSQ10 consisted of 18,219bp, with 71.9% G ＋ C content, encoding 17 open reading frames, 5 of them resembled those of Streptomyces plasmids. A rep locus （iteron within the gene） was identified for replication in Nocardiopsis sp. YIM 90083, and rep protein bound to its iteron sequence. This system may be useful for gene cloning and expression in Nocardiopsis.

关 键 词：NOCARDIOPSIS PLASMID REPLICATION 

分 类 号：Q782[生物学—分子生物学] TP393.08[自动化与计算机技术—计算机应用技术]