飞鱼籽生物酶法脱囊衣技术研究  

A method of taking off membranes with flying fish roes using enzyme preparation

作　　者：何贵山[1] 方旭波[1] 陈小娥[1] 余辉[1] 虞田[2] 

机构地区：[1]浙江海洋学院食品与药学学院,舟山316004 [2]南京农业大学食品科技学院,南京210095

出　　处：《食品科技》2011年第10期93-97,共5页Food Science and Technology

基　　金：浙江省科技厅重点科技专项农业项目(2009C12023);浙江省大学生科技创新项目

摘　　要：以飞鱼籽为原料,鱼籽脱囊衣率为指标,从胰蛋白酶、胶原蛋白酶、碱性蛋白酶和木瓜蛋白酶4种酶中,筛选出胰蛋白酶为最佳使用酶,并采用L9(34)正交实验设计对胰蛋白酶脱除飞鱼籽囊衣工艺参数进行优化。结果表明,酶解液浓度0.8mg/mL、pH9、在20℃下酶解12min,飞鱼籽脱囊衣率可达83%以上。Rate of taking off membranes of fish roes was used as an index with flying fish roes as the raw material,trypsin was screened out from trypsin,collagenase,alkaline protease and papain.The technological parameter of taking off membranes was optimized by L9（34） orthogonal experimental design,the result shows the optimal techniques obtainned are enzyme preparation amount 0.8 mg/mL,pH value 9,temperature 20 ℃ and enzymatic hydrolysis time 12 min.Rate of taking off membranes of flying fish roes 83% could be obtained.

关 键 词：飞鱼籽 脱囊衣 蛋白酶筛选 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]