同位素内标法测定欧李果汁游离氨基酸及成分分析  

Detection of free amino acids in Chinese dwarf cherry juice and composition analysis

作　　者：危晴[1] 刘俊英[1] 杨国伟[1] 王晓杰[1] 谢国莉[1] 

机构地区：[1]北京电子科技职业学院生物技术系,北京100029

出　　处：《食品科技》2011年第10期262-265,共4页Food Science and Technology

基　　金：北京市优秀教学团队课题资金支持

摘　　要：采用高效液相色谱-质谱联用(LC-MS/MS)同位素内标法,测定了欧李果原汁中游离氨基酸。结果表明,欧李果原汁中共检测出28种游离氨基酸,总含量为1640.1mg/L;人体必需8种氨基酸含量为86.8mg/L,婴幼儿必须氨基酸含量为16.2mg/L;支链氨基酸含量为34.6mg/L;脯氨酸含量为832.1mg/L;天门冬酰胺含量为300.5mg/L;γ-氨基丁酸含量为169.7mg/L。总结了脯氨酸、天门冬酰胺、γ-氨基丁酸营养价值,分析了其积累的原因。Free amino acids in Chinese dwarf cherry juice was determined with isotope internal standard method by high performance liquid chromatography-mass spectrometry（LC-MS/MS）.The main results show of the study were as follow.The Chinese dwarf cherry juice contains 28 free amino acids,the total content of its was 164.01 mg/100 mL;The one of 8 kinds of essential amino acids was 8.68 mg/100 mL;The content of amino acid for infant was 1.62 mg/100 mL;The one of branched-chain amino acid was 3.46 mg/100 mL,The one of proline content was 83.21 mg/100 mL;The one of asparagines was 30.05 mg/100 mL;The one of γ-amino butyric acid was 16.97 mg/100 mL.The nutritional value of proline,asparagines and γ-amino butyric acid were summarized,and the accumulation reasons for its were analyzed.

关 键 词：欧李果汁 游离氨基酸 同位素内标法 积累 

分 类 号：R151.2[医药卫生—营养与食品卫生学]