直投式乳酸菌剂对肥胖大鼠肝脏脂肪蓄积的影响研究  

The Effects of Direct Vat Set Lactobacillus Preparation on the Fat Accumulation in Liver of Obesity Rats

作　　者：石磊[1] 黄承钰[1] 张其圣[2] 徐家玉[1] 陈功[2] 王烨[1] 王卫东[1] 李鸣[1] 

机构地区：[1]四川大学华西公共卫生学院,成都610041 [2]四川省食品发酵工业研究设计院,成都611130

出　　处：《食品与发酵科技》2011年第5期1-5,共5页Food and Fermentation Science & Technology

基　　金：四川省重大科技支撑项目"优质安全中国泡菜现代产业关键技术研究与集成示范"(2009NZ0080-16)

摘　　要：[目的]研究直投式乳酸菌剂对肥胖大鼠肝脏脂肪蓄积的影响。[方法]60只雄性,SPF级SD大鼠适应性喂养3d后,按照体重随机分为5组,分别为对照组,肥胖模型组,低、中、高剂量直投式乳酸菌剂组,对照组和模型组分别喂饲基础饲料和高脂饲料,并灌胃蒸馏水,低、中、高剂量组喂饲高脂饲料并分别灌胃1.05×109cfu/kg/d,2.10×109cfu/kg/d,4.20×109cfu/kg/d的直投式乳酸菌悬液。每天记录各组大鼠的摄食情况,每周称体重,15周后,处死所有大鼠,取肝脏称重,计算肝体比;HE染色观察肝脏组织病理变化;并测定肝脏组织中TG、TC的含量。[结果]肥胖模型组大鼠平均每日能量摄入、体重、肝体比、肝脏中TG和TC含量均显著高于对照组(P<0.05,或P<0.01),高脂饲料成功造成了大鼠肥胖模型。与模型组相比,高剂量菌剂组大鼠平均膳食及能量摄入量均显著降低(P<0.01),而各实验组大鼠体重较模型组均无统计学差异;高剂量组大鼠的肝体比及三个实验组的肝脏TG显著低于肥胖组大鼠(P<0.01)。直投式乳酸菌实验组肝脏细胞脂肪变性以及炎性细胞的浸润减少。[结论]直投式乳酸菌剂能显著减少肥胖大鼠肝脏脂肪蓄积,具有一定抗脂肪肝作用。[Objective] the study was designed to investigate the effects of direct vat set lactobacillus preparation（DLP） on the fat accumulation in liver of obesity rats.[Methods] After 3 days adaptive feeding,a total of 60 SD rats were randomly divided into five groups,control group fed conventional diet,obesity model group fed high-fat diet,the low-,the middle-,and the high-dose experimental group fed high-fat diet and infused with the solution of DLP at levels of 1.05＊109cfu/kg/d,2.10＊109cfu/kg/d,4.20＊109cfu/kg/d,respectively.The food intake was recorded every day,and body weight was determined weekly.At 15 weeks,all rats were killed,and the liver was removed and liver/body weight ratio（LBR） was calculated;then the liver was sectioned for observing the change of liver pathology.The contents of TG and TC were also measured using commercial kits.[Results] the average energy intake,body weight,LBR,TG and TC in liver of obesity model group were significantly higher than that in controls（P0.05 or P0.01）,which suggested that the high-fat diet led to the obesity model in rats successfully.Compared with model group,the average food and energy intake decreased markedly in the high-dose group（P0.05）,and no statistical differences of body weight were observed between model group and experimental groups infused with DLP solution（P0.05）.The LBR in high-dose group and TG in three experimental groups reduced remarkably in comparison with the model group（P0.01）.The steatosis and inflammatory infiltration in liver of experimental groups were improved to some degrees.[Conclusion] The direct vat set lactobacillus preparation could reduce the fat accumulation in liver of obesity rats,and it might be applied to the prevention against fatty liver in the future.

关 键 词：直投式乳酸菌 肥胖 肝脏脂肪 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]