固相萃取-分光光度法快速检测腐竹中的碱性橙  

Rapid determination of basic orange in dried beancurd sticks by solid phase extraction-spectrophotometry

作　　者：李小燕[1,2] 仝海娟[1] 石展望[1] 罗少芹[1] 秦玉凤[1] 李梅[1] 

机构地区：[1]广西民族大学化学与生态工程学院,广西南宁530006 [2]广西林产化学品开发与应用重点实验室,广西南宁530006

出　　处：《食品工业科技》2011年第11期447-450,共4页Science and Technology of Food Industry

基　　金：广西自然科学基金项目(2010GXNSFA013047);广西教育厅科研项目(200911LX84)

摘　　要：目的:建立固相萃取净化富集,分光光度法快速检测腐竹中禁用色素碱性橙的方法。方法:样品经甲醇超声提取,C18固相萃取柱萃取,以20%甲醇溶液(V/V)为清洗剂净化,80%甲醇溶液(V/V)为洗脱剂洗脱富集后,用分光光度法进行测定。结果:碱性橙在2.0～10.0μg/mL浓度范围呈现良好的线性关系,相关系数R=0.9955;在2.0～8.0μg/mL添加水平时回收率范围为102.24%～84.15%,重复性实验平均回收率为98.45%,相对标准偏差为1.87%(n=5)。结论:该法灵敏度高、重复性好,操作简便快速,结果准确,适用于腐竹中碱性橙的快速检测。Objective：A novel method based on solid phase extraction followed by Spectrophotometry was developed for the analysis of prohibited colorant basic orange in dried beancurd sticks.Method：Basic orange in dried beancurd sticks was extracted with SIPORE SPE-C18SPE column,purified by 20% methanol aqueous solution（V/V）,eluted by 80% methanol aqueous solution（V/V）and detected by Spectrophotometry with mensurated at the maximum absorption wavelength 446nm.Results：The linear correlation coefficient was 0.9955 over the range of 2.0 ~ 10.0μg/mL.The recoveries were 102.24% ~ 84.15% for spiked standards at 2.0 ~ 8.0μg/mL level.The average recovery of repeated test was 98.45% with relative standard deviation（RSD）of 1.87 %（n=5）.Conclusions：This method with good linearity,high precision and rapidity was suitable for rapid determination of basic orange in dried beancurd sticks.

关 键 词：固相萃取 分光光度法 碱性橙 腐竹 

分 类 号：TS207.3[轻工技术与工程—食品科学]