酸酶法制备纳米豆渣纤维素  

The Preparation of Nanocrystalline Cellulose by Acid and Enzymatic Method

作　　者：刘玲玲[1] 田云波[1] 唐楚楚[1] 张宏[1] 刘雄[1] 

机构地区：[1]西南大学食品科学学院,国家食品科学与工程本科教学示范中心,重庆400715

出　　处：《食品与发酵工业》2011年第9期124-128,共5页Food and Fermentation Industries

基　　金：国家大学生创新性实验项目(091063524)

摘　　要：以富含纤维素的豆渣为原料,采用酸预处理粉碎后的豆渣,预处理条件为3 mol/L的HCl,水解温度100℃,水解时间120 min,HCl溶液添加量与原料比值为50∶1(mL∶g)。以酸预处理干燥后得到的样品作为纤维素酶水解的原料,通过单因素和正交实验,获得制备纳米纤维素的最佳酶解条件:酶用量3 000U/g,pH5.0,温度55℃,时间6 h,液料比20 mL/g。通过扫描电镜和透射电镜检测,制备出的纳米豆渣纤维素呈微球状,粒度为15～50nm。Nano-sized cellulose has excellent characters. The paper used soybean dregs which are rich in cellulose as raw materials. The grinding soybean dregs were pretreated by hydrochloric acid. The pretreatment conditions are 3 mol/L HCI in concentration, hydrolyzation temperature 100℃ , hydrolyzation time 120 min, acid water to material ratio 50： 1. The soybean -dreg cellulose pretreated with HCl and dried was hydrolyzed with cellulase. The optimum preparation technology of nano-sized soybean-dreg cellulose was investigated by single factor and orthogonal experiments： cellulase dosage 3 000U/g, pH 5.0, hydrolyzation temperature 55 ℃ , hydrolyzation time 6 h, water to material ratio 20 mL/g. The size of nanocrystalline cellulose with globosity appearance was 15 -50 nm through scanning electron microscope and transmission electron microscope.

关 键 词：豆渣 盐酸 纤维素酶 纳米纤维素 

分 类 号：TS209[轻工技术与工程—食品科学]