进出口食品中组胺的ELISA快速测定  

Rapid Detection of Histamine in Import and Export Food by ELISA Method

作　　者：郑海松[1] 杨小娇[2] 

机构地区：[1]安徽出入境检验检疫局,合肥230022 [2]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《食品工业》2011年第10期110-112,共3页The Food Industry

基　　金：出入境检验检疫行业标准项目(2009B165)

摘　　要：本文建立了一种进出口食品中组胺的酶联免疫快速测定方法。针对目前国内市场常见的Abraxis、r-Biopharm和Neogen三种试剂盒,进行比较选择最优试剂盒,并对酶联免疫分析定量检测组胺残留试剂盒进行选择性(交叉反应)测试、特异性与检测限测试以及对进出口食品样品组胺的回收率和精密度测试。结果显示,选择r-Biopharm试剂盒来检测样品中的组胺准确性较好,其对N-酰基-组胺交叉性为100%,对N-甲基-组胺、5-羟基吲哚乙酸、咪唑乙酸、L-组氨酸、N-甲基咪唑乙酸及血清素等组胺类似物交叉反应都小于1%,进出口食品中红葡萄酒的检测限(LOD)为250μg/kg,牛奶的LOD为100μg/kg,奶酪的LOD为2.5 mg/kg,鱼粉的LOD为100 mg/kg,样品的平均回收率在86.4%～95.6%,相对标准偏差2.7%～3.9%,经五个试验室的回收验证均具有较好的回收率,经HPLC确证假阳性率≤2.5%,表明酶联免疫分析定量法可快速、准确实现对食品中组胺残留量的快速筛选。To established an import and export food histamine by enzyme-linked immunosorbent fast determination method.According to the current domestic market common Abraxis,r-Biopharm and Neogen three kit,carries on the comparison to choose the optimum kit and enzyme-linked immunosorbent analysis quantitative detection of histamine residual kit for selective（cross reaction） testing,specificity and detection limit testing and samples of import and export food of histamine recovery rate and precision testing.The results showed that choose r-Biopharm kit to test samples of the histamine accuracy is better,which to N-Acyl-histamine cross is 100%,to N-Methyl-histamine,5-Hydroxy-indole-acetic acid,Imidazole acetic acid,L-Histidine,N-Methyl imidazole acetic acid and Serotonin cross reaction are less than 1%.Red wine detection limit（LOD） is 250 mg/kg,milk LOD is 100 mg/kg,cheese LOD is 2.5 mg/kg,fishmeal LOD is 100 mg/kg,the average recovery of sample in both 86.4%~ 95.6%,relative standard deviation 2.7%~3.9%.After five laboratory of recycling verify all have good recovery,by using HPLC corroborate false-positive rate not more than 2.5%.The results indicated that enzyme-linked immunosorbent analysis quantitative method can realize rapid and accurate in food histamine residues screening.

关 键 词：ELISA 组胺 食品 快速检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]