海洋活性酵母YS-185破壁技术的研究  

The Breaking Cell Wall Technologies of Marine Yeast YS-185

作　　者：郝建华[1] 王跃军[1] 刘均忠[1] 孙谧[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所海洋酶工程室,山东青岛266071

出　　处：《湖南农业科学》2011年第10期89-91,共3页Hunan Agricultural Sciences

基　　金：国家自然科学基金(30571429);国际科技合作重点项目计划(2005DFA30830)资助

摘　　要：采用酸碱法、珠磨法、酶溶法对海洋活性酵母YS-185进行破壁,并对其破壁的结果做了分析比较。结果表明:经酸碱法处理后的细胞破壁不明显,细胞基本保持原形态;珠磨法破碎程度较高,效果较好,但此种方法只适用于实验室规模的细胞破碎,且后处理工作复杂;酶溶法破壁率可达80%左右,效果较理想,在大规模生产中可以推广。The cell wall of marine yeast YS-185 was broken by three different technologies： acid-alkali method, bead- milling method and enzymatic lysis method, and theirs results were analyzed and compared. The results showed that the breakage effect of the cell wall treated with acid-alkali method was not obvious, and the yeast cells still kept their previous shape; the bead-milling method had the highest cytoclastic effect, but this method only fit for milling cell wall in laboratory for its complicated post-processing works; the cell wall breaking rate of the enzymatic lyses method could reach above 80%, and the effect was ideal. Therefore, enzymatic lysis method can be popularized in large scale production.

关 键 词：海洋酵母 破壁技术 酸碱法 珠磨法 酶溶法 

分 类 号：TS262.5[轻工技术与工程—发酵工程]