源气体流量比对氟化类金刚石薄膜蛋白吸附能力的影响  

Influence of source gas flow ratio on the proteins adsorbability of F-DLC film

作　　者：戴永丰[1] 江美福[1] 杨亦赏[1] 周杨[1] 

机构地区：[1]苏州大学物理科学与技术学院,苏州215006

出　　处：《物理学报》2011年第11期681-687,共7页Acta Physica Sinica

摘　　要：用316L不锈钢(SU316L)作基片,以高纯石墨作靶、CHF3和Ar作源气体,采用反应磁控溅射法在不同流量比R(CHF3/Ar)下制备了氟化类金刚石(F-DLC)薄膜.利用双蒸水液滴法、BCA(二喹啉甲酸)法和傅里叶红外光谱(FTIR)探讨了影响薄膜蛋白吸附能力的因素.结果表明,镀有F-DLC薄膜的SU316L表面的血小板黏附量明显减少,血小板的变形程度显著减轻,相应的白蛋白与纤维蛋白原的吸附比普遍高于未镀膜的SU316L表面的相应值,说明镀上F-DLC薄膜可以改善样品的血液相容性.流量比为2:1左右时制备出的F-DLC薄膜,其白蛋白与纤维蛋白原的吸附比值达到最大,相应的血液相容性最佳.对薄膜的接触角和表面能以及FTIR光谱分析研究表明,SU316L表面的F-DLC薄膜的白蛋白与纤维蛋白原的吸附比及血液相容性与薄膜的中的—CFx键的含量(F/C比)和表面能(疏水性)直接相关,控制源气体流量比可以实现对薄膜的血液相容性的调制.The fluorinated diamond-like carbon （F-DLC） films are prepared by reactive magnetron sputtering under different gas flow radios with trifluoromethane （CHF3） and argon （Ar） used as source gases and pure graphite as a target on the surface of 316L stainless steel （SU316L）.Factors which influence the protein adsorbability are discussed by double-stilled water,BCA and FTIR spectra.The results show that the surface of SU316L coated with F-DLC film could obviously reduce the number of adherent platelets and dramatically relieves the deformation of platelets,leading to a ratio of higher albumin to fibrinogen adsorption higher than that with using the SU316L substrates,which indicates that the SU316L coated with F-DLC film can improve the blood compatibility.The film has the highest ratio of albumin to fibrinogen adsorption and the best hemocompatibility when the ratio of gas flow is 2∶1.Furthermore,the measurements of the contact angle,the surface energy of films and FTIR spectra show that the ratio of albumin to fibrinogen adsorption and the hemocompatibility of F-DLC coated SU316L depend on the surface energy （hydrophobic nature） of films and the quantity of —CFx bonds （the ratio of F/C） contained in film.The modulating of blood compatibility of the films can be realized by the control of the ratio of source gas flow.

关 键 词：反应磁控溅射 氟化类金刚石薄膜 蛋白吸附 

分 类 号：R318.08[医药卫生—生物医学工程] TN304.055[医药卫生—基础医学]