纳米硒对岢岚绒山羊妊娠母羊及胎儿抗氧化性、硒蛋白表达及生长发育的影响  

Effects of Nano-Selenium on Antioxidation Function,Selenoprotein Expression,Growth and Development of Pregnant Cashmere Goat and Fetus in KeLan

作　　者：武晓英[1] 曹贵东 任有蛇[3] 岳文斌[3] 

机构地区：[1]太原师范学院生物系,山西太原030031 [2]山西新福瑞沃生物技术工程有限责任公司,山西太原030012 [3]山西农业大学动物科技学院,山西太谷030801

出　　处：《激光生物学报》2011年第5期631-638,共8页Acta Laser Biology Sinica

基　　金：国家科技支撑计划子项目(2007BAD56B03-2)

摘　　要：为研究纳米硒对岢岚绒山羊妊娠母羊及胎儿抗氧化能力、硒蛋白表达和生长发育的影响。选择体重相近的岢岚妊娠绒山羊80只,随机分为2组,分别喂以基础日粮和添加0.5mg/kg DM纳米硒的饲粮,实验期110d。结果表明:日粮中纳米硒的添加极显著(P<0.01)地提高了母羊及胎儿血清中GSH-Px、SOD的活性;极显著(P<0.01)提高了母羊及胎儿肝、胎盘组织中GSH-Px、SOD的活性;极显著(P<0.01)降低了母羊及胎儿血清与胎盘组织中MDA的浓度。在日粮中添加纳米硒后,母羊及胎儿肝、肾组织中cGPx1 mRNA的表达显著提高(P<0.05);胎盘组织中的cGPx、SeP、Trx1 mRNA的表达极显著提高(P<0.01)。妊娠母羊的基础日粮中加入纳米硒,极显著(P<0.01)提高了妊娠母羊及胎儿血清、肝、胎盘组织中IGF-1的含量和IGF-1mRNA表达量,显著增加了胎盘、胎儿及胎儿肝、肾组织的重量(P<0.05)。日粮中添加0.5mg/kg DM纳米硒,增强了妊娠母羊及胎儿的抗氧化能力,提高了IGF-1、cGPx1、SeP和Trx1 mRNA的表达量,进一步促进了胎儿的生长发育。The study was conducted to investigate the effects of Nano-Se on antioxidation, selenoprotein expression, growth and development of pregnant cashmere goat and fetus in KeLan. A total of 80 pregnant cashmere goats in KeLan with approximately equal body weight were allotted to one of two experimental treatments, a control （ C, n = 40） without supplemental Se in basal diet or supplemented （ S, n = 40） with nano-selenium （ declared Se content O. 5 mg/kg DM）. The whole feeding experiment lasted for 110 days. The results from supplemental Nano-Se in diet showed that activities of glutathione peroxidase （GSH-Px） and superoxide dismutase （ SOD ） were markedly increased （ P 〈 0.01 ） in serum of pregnant goats and fetus, activities of GSH-Px and SOD were markedly increased （P 〈 0.01 ） in live, placental tissues of pregnant goats and fetus, malondialdehyde （MDA） concentration were markedly decreased （ P 〈 0.01 ） in serum of pregnant goats and fetus and placental tissue. Adding Nano-Se in diet, Expressions of cGPx1mRNA were enhanced significantly（P 〈 0.05） in live, kidney tissues of pregnant goats and fetus. The mRNA expressions of SeP, Trxl were markedly enhanced （P 〈 0.01 ） in placental tissues of pregnant goats and fetus. Supplementation of basal diet with Nano-Se markedly increased the concentration of 1GF-1 and expression of IGF-I mRNA （ P 〈 0.01 ） in serum and tissues of pregnant goat and fetus. Compared with basal diet group, weight of placenta, fetus, fetal liver and kidney tissues increased signifieandy （P 〈 0.05 ）. It is concluded that supplementation of Nano-Se can improve the antioxidation function, increase the mRNA expressions of cGPxl, SeP, Trxl and 1GF-1, and increase growth development of fetus. Supplementation level of Nano-Se is 0.5 mg/kg DM in basal diet for pregnant goats.

关 键 词：纳米硒 抗氧化性 硒蛋白 绒山羊 

分 类 号：S858.27[农业科学—临床兽医学] Q522.2[农业科学—兽医学]