棉籽分离蛋白的盐法制取研究  

PREPARATION OF COTTONSEED PROTEIN ISOLATE BY AQUEOUS SALT SOLUTION METHOD

作　　者：管军军[1] 何旭[1] 贾丰巧[1] 

机构地区：[1]河南工业大学生物工程学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2011年第5期11-15,31,共6页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南工业大学引进人才专项基金(150220)

摘　　要：通过盐法制取棉籽分离蛋白,对其提取工艺、亚基组成及脱酚进行了研究.响应面试验结果表明:盐法提取棉籽蛋白最佳工艺条件为w(NaCl)2.6%、温度37℃、时间2.5 h,提取率可以达到56.9%(±0.3%);SDS-PAGE分析发现所制取的棉籽分离蛋白富含中高相对分子质量亚基(27 000～35 000),而高相对分子质量亚基(45 000～50 000)含量甚少,这与NaOH法相反;经过甲醇洗脱后,盐法提取的棉籽蛋白中棉酚含量大幅减少,其残留可达到相关国际标准.In the paper,we prepared the cottonseed protein isolate（CPI） by the aqueous salt solution method andstudied the protein extraction process,subunit composition and gossypol removal process.Response surfaceanalysis results showed that the optimum salt extraction conditions of cottonseed protein were： NaCl massconcentration 2.6%,temperature 37 ℃ and time 2.5 h,and the extraction rate could reach 56.9%（±0.3%）.SDS-PAGE results indicated that the prepared CPI was rich in high-molecular weight subunits（27 000~35 000）,but contained few higher-molecular weight subunits（45 000~50 000）,which was in contrast with the NaOHextraction.After methanol elution,gossypol content of the CPI prepared by the salt extraction was substantiallyreduced,and its residue in the protein could attain the relevant international standards.

关 键 词：棉籽分离蛋白 盐法 提取工艺 亚基 棉酚 

分 类 号：TS201.2[轻工技术与工程—食品科学]