气相色谱-质谱法测定食品中2,4-滴丁酸的残留量  

Determination of 2,4-Dichlorophenoxybutyric Acid Residue in Foods using Gas Chromatography-Mass Spectrometry

作　　者：唐食明[1] 冯家望[1] 吴洁珊[1] 刘李登[1] 余以刚[2] 

机构地区：[1]珠海出入境检验检疫局技术中心,广东珠海519015 [2]华南理工大学轻工与食品学院,广东广州510640

出　　处：《现代食品科技》2011年第11期1410-1413,1418,共5页Modern Food Science and Technology

摘　　要：建立了检测8种动物源性和植物源性食品中2,4-滴丁酸(2,4-Dichlorophenoxybutyric acid,2,4-DB)残留量的气相色谱-质谱法。试样经乙腈-水提取,Oasis MAX阴离子交换小柱净化,经双(三甲基硅烷基)三氟乙酰胺衍生化,气相色谱质谱法检测,外标法定量。方法中2,4-DB在0.01μg/mL^5.0μg/mL浓度范围内线性关系良好(r>0.99);0.1 mg/kg、0.2 mg/kg、1.0 mg/kg加标水平的平均回收率为73%~98.3%,方法的总体变异系数为2.8%~5.8%;方法检出限为0.1 mg/kg。A method for determination of 2,4-Dichlorophenoxybutyric acid by gas chromatography-mass spectrometry（GC-MS） was developed.The sample was extracted with acetonitrile-water,purified by Oasis MAX column,derivatizated by bis（trimethylsilyl） trifluoroacetamide,and analyzed by GC-MS,quantified by external standard method.The linear relationships was fine（r0.99） with a linear range from 0.01 to 5.0 μg/mL.The mean recovery were 73%~98.3% when 2,4-DB was added at levels of 0.1 mg/kg,0.2 mg/kg,1.0 mg/kg,the residual standard deviation between the sample determinations was between 2.8%~5.8%.The detection limit of the residues was 0.1 mg/kg which meet the international determination request of 2,4-Dichlorophenoxybutyric acid residues.

关 键 词：气相色谱-质谱法 2 4-滴丁酸 衍生化 固相萃取 食品 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]