Cloning and Prokaryotic Expression of VP1 Gene of Foot-and-Mouth Disease Virus (FMDV) Type O  

O型口蹄疫病毒结构蛋白基因VP1的克隆与原核表达(英文)

作　　者：付薇[1,2] 陈磊[1] 熊毅[2] 潘琼[1] 王常伟[1] 陈进喜[1] 胡晓静[1] 刘棋[2] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005 [2]广西动物疫病预防控制中心,广西南宁530001

出　　处：《Agricultural Science & Technology》2008年第5期55-58,154,共5页农业科学与技术（英文版）

基　　金：Supported by the Science and Technology Foundation from Science&Technology Department of Guangxi Autonomous Region(0779001)~~

摘　　要：According to the complete genome of foot-and-mouth disease virus(FMDV)type O,a pair of special primers was designed to amplify VP1 gene.The VP1 gene was amplified by RT-PCR and subsequently inserted into the expression vector pGEX-6p-1 and induced by IPTG.Then SDS-PAGE showed the expressed protein was 51 kD in molecular weight.Then the product was purified by GSTrap FF columns.The product was detected through Western-blot that showed the protein has antigenicity.It provided fundamental data and materials for further investigation on diagnosis method of FMDV.According to the complete genome of foot-and-mouth disease virus(FMDV)type O,a pair of special primers was designed to amplify VP1 gene.The VP1 gene was amplified by RT-PCR and subsequently inserted into the expression vector pGEX-6p-1 and induced by IPTG.Then SDS-PAGE showed the expressed protein was 51 kD in molecular weight.Then the product was purified by GSTrap FF columns.The product was detected through Western-blot that showed the protein has antigenicity.It provided fundamental data and materials for further investigation on diagnosis method of FMDV.

关 键 词：Foot-and-mouth disease virus Structural protein VP1 CLONING Expression 

分 类 号：S852.65[农业科学—基础兽医学]