CONSTRUCTION OF A GENOMIC DNA LIBRARY WITH ATA VECTOR AND ITS APPLICATION IN CLONING OF THE PHYTOENE SYNTHASE GENE FROM THE CYANOBACTERIUM SPIRULINA PLATENSIS M-135  

作　　者：Yoshikazu Kawata Shin-ichi Yano Hiroyuki Kojima(Osaka Mational Research Institute. Agency of Industrial Science and Technology, Ikeda, Osaka 563, Japan) 

出　　处：《Chinese Journal of Oceanology and Limnology》1998年第S1期17-24,共8页中国海洋湖沼学报（英文版）

摘　　要：An efficient and simple method for constructing a genomic DNA library using a TAcloning vector is presented. It is based on the sonicative cleavage of genomic DNA and modification offragment ends with Taq DNA polymerase, followed by ligation using a TA vector. This method was ap-plied for cloning of the phytoene synthase gene crtB from Spirulina platensis. This method is useful whengenomic DNA cannot be efficiently digested with restriction enzymes, a problem often encountered duringthe construction of a genomic DNA library of cyanobacteria.An efficient and simple method for constructing a genomic DNA library using a TAcloning vector is presented. It is based on the sonicative cleavage of genomic DNA and modification offragment ends with Taq DNA polymerase, followed by ligation using a TA vector. This method was ap-plied for cloning of the phytoene synthase gene crtB from Spirulina platensis. This method is useful whengenomic DNA cannot be efficiently digested with restriction enzymes, a problem often encountered duringthe construction of a genomic DNA library of cyanobacteria.

关 键 词：GENOMIC library SPIRULINA PHYTOENE SYNTHASE CAROTENOID 

分 类 号：Q78[生物学—分子生物学]