Effects of blocking Le^Y oligosaccharide on cell surface to MMPs secreted by blastocysts and epithelial cells in mouse in vitro  

作　　者：Xiaoqi Wang Yu Liu Enkui Duan Guoqing Zeng Zhengmei Zhu 

机构地区：[1]Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China [2]Dalian Med Univ, Dept Biochem, Dalian 116027, Peoples R China

出　　处：《Chinese Science Bulletin》1998年第17期1461-1465,共5页

基　　金：ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .39470 170 )andStateKeyLaboratoryofReproductiveBiology ,InstituteofZoology ,theChineseAcademyofSciences

摘　　要：In order to understand the role of Le+Y oligosaccharide antigen (Le+Y) during implantation, the relationship of Le+Y on the cell surface with matrix metalloproteinase (MMPs) secreted by blastocysts and monolayer epithelial cells during implantation in the mouse %in vitro% was studied by monoclonal antibody (mAb) AH-6, directed to Le+Y[Fuc α1-2 Gal β1-4 (Fuc α1-3) GlcNAc-], and gelatin zymography. The results showed that MMPs secretion was reduced after Le+Y on the cell surface of either epithelial cells or trophoblasts was blocked. It indicated that MMPs expression which played an important function during the process of implantation were regulated by Le+Y. Therefore, it was considered that Le+Y could regulate embryos invasion by some mechanism.In order to understand the role of LeY oligosaccharide antigen (LeY) during implantation, the relationship of LeY on the cell surface with matrix metalloproteinase (MMPs) secreted by blastocysts and monolayer epithelial cells during implantation in the mousein vitro was studied by monoclonal antibody (mAb) AH6, directed to LeY[Fuc α1-2 Gal ?1-4 (Fuc αl-3) GIcNAc-], and gelatin zymography. The results showed that MMPs secretion was reduced after LeY on the cell surface of either epithelial cells or trophoblasts was blocked. It indicated that MMPs expression which played an important function during the process of implantation were regulated by LeY. Therefore, it was considered that LeY could regulate embryos invasion by some mechanism.

关 键 词：MOUSE Le+Y OLIGOSACCHARIDE antigen matrix METALLOPROTEINASES implantation MONOCLONAL antibody. 

分 类 号：Q45[生物学—生理学]