Folding patterns of chromatin in chicken erythrocyte studied by using AFM and TEM  

作　　者：Liu, ZX Zhou, MY Xie, HY Qian, RL Zhang, Y Hu, J Li, MQ 

机构地区：[1]Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China [2]Chinese Acad Sci, Shanghai Inst Nucl Res, Shanghai 201800, Peoples R China

出　　处：《Chinese Science Bulletin》1998年第4期289-291,共3页

基　　金：ThisworkwassupportedbytheFoundationofChineseAcademyofSciences (GrantNo .KJ95 1_Al_6 0 3)andShanghaiOpenLaboratoryofLifeSciences.

摘　　要：Four folding patterns (～15, ～30-40, ～60 and ～90-110 nm) of the higher_order structure of chromatin in chicken erythrocytes have been observed with an atomic force microscope (AFM) under near natural conditions. Results showed that the chromatin in the interphase nucleus of chicken erythrocyte was not in a disorganized form but represented a higher_order conformation. The thin sections of the chromatin in the nucleus of chicken erythrocyte were examined with a transmission electron microscope (TEM). Compared with TEM, AFM had the advantages that the samples did not need the preparation procedures such as fixing, sectioning and staining, and thus were closer to their native condition.Four folding patterns (similar to 15, similar to 30-40, similar to 60 and similar to 90-110 nm) of the higher-order structure of chromatin in chicken erythrocytes have been observed with an atomic force microscope (AFM) under near natural conditions. Results showed that the chromatin in the interphase nucleus of chicken erythrocyte was not in a disorganized form but represented a higher-order conformation. The thin sections of the chromatin in the nucleus of chicken erythrocyte were examined with a transmission electron microscope (TEM). Compared with TEM, AFM had the advantages that the samples did not need the preparation procedures such as fixing, sectioning and staining, and thus were closer to their native condition.

关 键 词：CHROMATIN structure NUCLEUS of CHICKEN ERYTHROCYTE AFM TEM. 

分 类 号：S813[农业科学—畜牧学]