REGULATION OF STEROIDOGENESIS OF INFANT AND ADULT RHESUS MONKEY GRANULOSA CELLS in vitro  

作　　者：刘以训 胡召元 林晓 邹如今 

机构地区：[1]Laboratory of Reproductive Biology, Institute of Zoology, Academia Sinica, Beijing 100080, PRC

出　　处：《Science China Chemistry》1990年第9期1060-1069,共10页中国科学（化学英文版）

基　　金：This work was supported by the National Natural Science Foundation of China and the Rockefeller Foundation; New York.

摘　　要：The present studies have demonstrated that infant monkey granulosa cells, like the adult ones, have the potential of responding markedly in vitro to human FSH, cyclic-AMP and forskolin, resulting in the increase of progesterone and estrogen production. Exogenous hCG was also capable of increasing FSH-stimulated progesterone biosynthesis in both infant and adult granulosa cells, but did not stimultate the infant granulosa cells to secrete estrogen. Addition of a synthetic estrogen, diethylstilestrol, to the culture of monkey granulosa cells enhanced the FSH-stimulated progesterone and estrogen production. The esteroidogenesis of monkey granulosa cells was also dramatically stimulated by a gonadotropin-releasing hormone agonist. Monkey granulosa cells, unlike the other animal cells, secrete measurable amount of estrogen in the absence of androgen substrate. The findings reported here are significant in regard to understanding of the mechanism of hormonal regulation of primate ovarian function.The present studies have demonstrated that infant monkey granulosa cells, like the adult ones, have the potential of responding markedly in vitro to human FSH, cyclic-AMP and forskolin, resulting in the increase of progesterone and estrogen production. Exogenous hCG was also capable of increasing FSH-stimulated progesterone biosynthesis in both infant and adult granulosa cells, but did not stimultate the infant granulosa cells to secrete estrogen. Addition of a synthetic estrogen, diethylstilestrol, to the culture of monkey granulosa cells enhanced the FSH-stimulated progesterone and estrogen production. The esteroidogenesis of monkey granulosa cells was also dramatically stimulated by a gonadotropin-releasing hormone agonist. Monkey granulosa cells, unlike the other animal cells, secrete measurable amount of estrogen in the absence of androgen substrate. The findings reported here are significant in regard to understanding of the mechanism of hormonal regulation of primate ovarian function.

关 键 词：RHESUS MONKEY GRANULOSA cell steroidogenesis SERUM-FREE culture 

分 类 号：O6[理学—化学]