SERUM-FREE

作品数：20被引量：45H指数：4
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相关领域：医药卫生生物学更多>>
相关作者：魏续仓赵文理李梅生杨娣娣王彤更多>>
相关机构：陕西省人民医院第二军医大学中国科学院广州生物医药与健康研究院上海交通大学医学院附属新华医院更多>>
相关期刊：《药学服务与研究》《World Journal of Stem Cells》《Journal of Integrative Agriculture》《化工学报》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划广东省自然科学基金更多>>
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Efficacy equivalence but hidden hurdles:Can serum-free human umbilical cord mesenchymal stem cells translate to clinically superior osteoarthritis therapy
《World Journal of Stem Cells》2025年第3期144-146,共3页Fang Lin Ke-Xin Ma Yue Ding Xiao-Ting Liang 
Supported by the Natural Science Foundation of Shanghai,No.24ZR1459300(to Xiao-Ting Liang);and the Pyramid Talent Project,No.YQ677(to Yue Ding).
This article discusses the study by Xiao et al,which investigated the therapeutic efficacy of serum-free cultured human umbilical cord mesenchymal stem cells(NhUCMSCs)in a mouse model of knee osteoarthritis.The result...
关键词：Mesenchymal stem cells Serum-free culture OSTEOARTHRITIS Cartilage repair Regenerative medicine 
Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells被引量：3
《Bioactive Materials》2021年第12期4377-4388,共12页Bin Jiang Weijie Li Samantha Stewart Wenquan Ou Baolin Liu Pierre Comizzoli Xiaoming He 
This work was partially supported by grants from the US National Science Foundation(CBET-1831019 to XH and DGE-1840340 that is a GRFP fellowship to SS);National Institutes of Health(NIH R01EB023632 to XH).
Human induced pluripotent stem cells(hiPSCs)possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use.However,contempor...
关键词：SAND Ice seeding CRYOPRESERVATION iPSC Stem cell 
Biological characterization of human amniotic epithelial cells in a serum-free system and their safety evaluation被引量：13
《Acta Pharmacologica Sinica》2018年第8期1305-1316,共12页Peng-jie YANG Wei-xin YUAN Jia LIU Jin-ying LI Bing TAN Chen QIU Xiao-long ZHU Cong QIU Dong-mei LAI Li-he GUO Lu-yang YU 
This work was supported by the Project of Health Collaborative Innovation of Guangzhou City （Grant No 201704020214）, the National Natural Science Foundation of China （Grant No 81770444 and 81600354） and Fundamental Research Funds for the Central Universities of China.
Human amniotic epithelial cells （hAECs）, derived from the innermost to be potential seed cells for allogeneic cell therapy. Previous studies ayer of the term placenta closest to the fetus, have been shown have shown...
关键词：human amniotic epithelial cells cell therapy safety evaluation SERUM-FREE 
Novel sericin-based hepatocyte serum-free medium and sericin's effect on hepatocyte transcriptome被引量：1
《World Journal of Gastroenterology》2018年第30期3398-3413,共16页Yun Huang Qing Peng Hai-Yan Li Zhi-Dong Jia Yang Li Yi Gao 
Supported by the National Natural Science Foundation of China,No.81470875;the Natural Science Foundation of Guangdong Province,No.2014A030312013;Science and Technology Planning Project of Guangdong Province,No.2014B020227002,No.2015B090903069,and No.2015B020229002;Science and Technology Program of Guangzhou,No.201604020002
AIM To develop a novel hepatocyte serum-free medium based on sericin,and to explore the effect of sericin on the hepatocyte transcriptome.METHODS A controlled trial comparing novel serum-free medium and other media: C...
关键词：SERICIN SERUM-FREE medium MAPK pathway Bioartificial liver support system CCR6 
人原代角质形成细胞在两种培养基中增殖能力的比较
《药学服务与研究》2017年第2期114-119,共6页陶丛珊 薛潇春 兰芬 唐炜雅 李超 胡晋红 
目的:比较第一代和第二代人原代角质形成细胞(human primary keratinocytes,HPK)在两种培养基[(EpiLife培养基+HKGS)和DK-SFM培养基]中增殖能力的差异。方法:通过两步法从人的皮肤组织获得第一代HPK,进行传代后获得第二代HPK,采用细胞...
关键词：角质形成细胞 细胞培养技术 EpiLife培养基 defined-keratinocyte serum-free培养基 
Ex vivo expansion of hematopoietic stem and progenitor cells: Recent advances
《World Journal of Hematology》2014年第2期18-28,共11页Katsuhiro Kita Fangming Xiu Marc G Jeschke 
Supported by Canadian Institutes of Health Research,No.123336;CFI Leader’s Opportunity Fund,No.25407;Physicians’ Services Incorporated Foundation-Health Research Grant Program(MGJ)
Hematopoietic stem cells(HSCs) have become the most extensively studied stem cells and HSC-based cellular therapy is promising for hematopoietic cancers and hereditary blood disorders. Successful treatment of patients...
关键词：HEMATOPOIETIC STEM CELL Ex vivo expansion SERUM-FREE culture CELL surface MARKERS ENRICHMENT STEM CELL isolation 
ALK Family Inhibitor A83-01 Promotes the Proliferation of Mouse Male Germline Stem Cells (mGSCs) Under Serum- and Feeder-Free Conditions
《Journal of Integrative Agriculture》2013年第10期1839-1846,共8页YU Meng WANG Long HU Yue LIAN Zhi-min HUA Jin-lian 
supported by grants from the National Natural Science Foundation of China, China (30972097, 31272518);the Program for New Century Excellent Talents in University, China (NCET-09-0654);the Fundamental Research Funds for the Central Universities, China (QN2011012)
A83-01 is a selective inhibitor of the TGF-β type I receptor ALK,which inhibits the TGF-β-induced epithelial-to-mesenchymal transition（EMT） via the inhibition of Smad2 phosphorylation.Previous studies have showed ...
关键词：male germline stem cells（mGSCs） A83-01 feeder-and serum-free mouse 
Development and quality of bovine embryos produced in vitro using growth factor supplemented serum-free system
《Open Journal of Animal Sciences》2011年第3期97-105,共9页Arindam Dhali Vahida M. Anchamparuthy Steve P. Butler Isis K. Mullarky Ronald E. Pearson Frank C. Gwazdauskas 
The influence of a growth factor supplemented serum-free system on the development, gene expression, and cryotolerance of in vitro pro- duced bovine embryos was investigated. To assess the embryo development and gene ...
关键词：IVF Growth Factors EMBRYO DEVELOPMENT Gene Expression CRYOTOLERANCE BOVINE 
Isolation and Identification of Cancer Stem Cells from Human Osteosarcom by Serum-free Three-dimensional Culture Combined with Anticancer Drugs被引量：7
《Journal of Huazhong University of Science and Technology(Medical Sciences)》2010年第1期81-84,共4页周松 李锋 肖骏 熊伟 方忠 陈文坚 牛鹏彦 
The cancer stem cells(CSCs)from human osteosarcoma by serum-free three-dimensional culture combined with anticancer drugs were isolated and identified.The primary cells derived from human osteosarcoma were digested by...
关键词：three-dimensional culture serum-free culture EPIRUBICIN OSTEOSARCOMA cancer stem cells 
Serum-free culture of H pylori intensifies cytotoxicity被引量：2
《World Journal of Gastroenterology》2007年第4期532-537,共6页Hiroyuki Ohno Akiyuki Murano 
AIM： To perform a long culture passage of H pylori without serum, taking into account its cytotoxicity and the presence of the probable new cytotoxic factor. METHODS： One sample of H pylon 60190 （ATCC 49503） was g...
关键词：H pylori CYTOTOXICITY Serum-free longculture 
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