钛胶反相高效液相色谱法测定饮料中的咖啡因  

Determination of caffeine in soft drinks by titania-based based reverse phase high performance liquid chromatography

作　　者：包荣[1] 李荣[1] 姜子涛[1] 

机构地区：[1]天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室,天津300134

出　　处：《中国食品添加剂》2011年第5期214-218,共5页China Food Additives

基　　金：国家自然科学基金项目(项目编号:20875069)

摘　　要：建立了利用钛胶反相柱快速测定饮料中咖啡因的含量的高效液相色谱方法。色谱条件:分离柱为Titania Sachtopore-RP柱(250mm×4.6mm i.d.,5μm),柱温30℃,流动相为水:甲醇=83:17(V/V),流速1mL/min,检测波长273nm。线性范围为12-900μg/mL,此范围内标准曲线线性良好(R2=0.9998)。最低检出限(S/N=3)为0.03μg/mL,样品加标回收率范围为98.6%～102.2%,精密度即相对标准偏差(n=9)小于0.23%。本法简单易操作、分析速度快,精密度和准确度均取得满意效果,适用于各种饮料样品中咖啡因的定量分析。本研究不仅是对食品中咖啡因检测方法的补充,更重要的是发挥了钛胶固定相不可比拟的优势。A rapid method for the determination of caffeine in soft drinks by titania-based reverse phase high performance liquid chromatography was reviewed in this paper.The chromatographic separation conditions were as follow：a Titania Sachtopore-RP separated column（250×4.6 mm,5μm）was used and column temperature was 30°C;the mobile phase was a mixture of water and methanol in the ratio of 83 to 17（V/V）;the flow rate was 1 mL/min.The detection wavelength was 273nm.The method presented good linearity（R2=0.9998）toward caffeine in the range of 12~900μg/mL.The limit of detection（S/N=3）for caffeine was 0.03μg/mL.The recovery of spiked caffeine in samples ranged from 98.6%~102.2%.The precision（RSD）for 9 replicate detections was less than 0.23%.The method is simple operation,quick analysis,accurate and precise.Meanwhile,it is applicable to the quantitative analysis of caffeine in drinks.This study is not only a supplement to the detecting methods for caffeine,but also takes the advantages of titania-based stationary phase.

关 键 词：咖啡因 钛胶柱 高效液相色谱法 饮料 

分 类 号：TS207.3[轻工技术与工程—食品科学]