Folin-Ciocalteu分光光度法测定茶多酚锌配合物中多酚的含量  

Determination of total polyphenols in the zinc complex of polyphenol by Folin-Ciocalteu colorimetry

作　　者：王彦兵[1] 胥艳[1] 魏永春[2] 刘小强[1] 刘绣华[1,3] 

机构地区：[1]河南大学化学化工学院,河南开封475004 [2]北京理工大学珠海学院化工与材料学院,广东珠海519085 [3]河南大学河南省天然药物与免疫工程重点实验室,河南开封475004

出　　处：《化学研究》2011年第6期76-78,共3页Chemical Research

基　　金：河南省国际合作项目(No.084300510023)

摘　　要：以没食子酸为对照品,采用Folin-Ciocalteu比色法测定了茶多酚锌配合物(TP-Zn)中多酚的含量.结果表明,多酚质量浓度在0～8.54mg.L-1范围内与吸光度呈良好的线性关系;稳定性、精密度、重现性和回收率实验的相对标准偏差在0.39%～1.23%范围内.该法是一种简便、快速、准确测定茶叶制品中多酚含量的可靠方法.A quantitative determination method for total polyphenols content in the Zinc complex of polyphenol was developed by using Folin-Ciocalteu colorimetry with gallic acid as reference compound.The results showed that the linear range of calibration curve was 0-8.54 mg·L-1.The RSD（relative standard deviation） of stability,precision,repeatability and recovery rate were in the range of 0.39%-1.23%.Therefore,FC method is a convenient,fast and accurate method to determine the total polyphenols of tea products.

关 键 词：茶多酚 锌配合物 多酚 Folin-Ciocalteu比色法 

分 类 号：O657.32[理学—分析化学]