高效液相色谱原子荧光分光光度联用法测定海产品中的甲基汞含量  

Determination of methylmercury in seafood by HPLC coupled with hydride generation and AFS

作　　者：赵凯[1] 杨大进[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2011年第6期534-539,共6页Chinese Journal of Food Hygiene

摘　　要：目的建立高效液相色谱原子荧光分光光度法在线联用技术测定海产品中甲基汞的方法。方法以25%氢氧化钾甲醇溶液水浴加热后超声提取样品,试液中的甲基汞与2-巯基乙醇结合。以5%甲醇溶液(含60 mmol/L乙酸铵和0.1%2-巯基乙醇)作流动相,经Supelco C18色谱柱(150 mm×4.6 mm,5μm)分离,紫外消解后经KBH4还原由原子荧光光度计进行测定。结果甲基汞的检出限为0.7μg/L(以汞计),样品测定的相对标准偏差小于4.6%,采用两种参考物质考查方法的准确性,以测定市售海产品中甲基汞含量。结论本研究通过简化仪器装置,改进前处理步骤,有效地提高了方法的可靠性,该方法简便、快速、可靠,可用于海产品中甲基汞的含量测定。Objective A high performance liquid chromatography（HPLC） coupled with hydride generation and atomic fluorescence spectrometry is proposed for the determination of methyl mercury in seafood.Methods Samples were extracted by 25% potassium hydroxide and the methylmercury-thiol complex was separated on a Supelco C18（150 mm×4.6 mm,5 μm） reverse phase column and online UV-digested.After reduced to element Hg by KBH4,the element Hg was selectively detected by AFS in an Ar/H2 flame under optimized conditions.Results The detection limit for methyl mercury was 0.7 μg/L（as mercury） with a relative standard deviation（RSD） of lower than 4.6%.Two standard reference materials were applied for the accuracy of study.Conclusion The content of methylmercury in seafood can be determined by the method with good convenience,rapidity and reliability.

关 键 词：海产品 甲基汞 高效液相色谱法 原子荧光光度法 联用技术 食品污染物 食品安全 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] O657.31[轻工技术与工程—食品科学与工程]