玉米胚芽分离蛋白提取率及氨基酸组分分析  

Extraction rate of protein isolate in corn germ and its amino acid composition

作　　者：魏旭[1] 肖志刚[1] 冯秋娟[1] 郑广钊[1] 叶鸿剑[1] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030

出　　处：《东北农业大学学报》2011年第11期51-57,共7页Journal of Northeast Agricultural University

基　　金：哈尔滨市青年科学技术专项基金(2007RFQXN019)

摘　　要：利用水、氯化钠、乙醇、氢氧化钠,分别提取玉米胚芽中的水溶蛋白、盐溶蛋白、醇溶蛋白、碱溶蛋白,确定各种分离蛋白的最佳溶剂条件。样品过80目筛后,脱脂处理,取5 g脱脂样品进行试验。结果表明,水溶蛋白的最佳溶剂量为水80 mL,提取率为37.13%;盐溶蛋白的最佳溶剂浓度为质量分数为6%的氯化钠溶液,提取率为25.34%;醇溶蛋白的最佳溶剂浓度为质量分数为70%的乙醇溶液,提取率为3.8%;碱溶蛋白的最佳溶剂浓度为质量分数为0.3%的氢氧化钠溶液,提取率为40.18%。此外对蛋白的氨基酸组分进行了分析。This paper aims to find out the best solvent conditions for extracting protein by sequentially separating the water-soluble,salt-soluble,prolamin-soluble and alkali-soluble protein in corn germ,using water,NaCl,ethanol and NaOH as solution.Take 5 g sample from defatted corn germ of 80 mesh to do the research.And the result showed that the optimal amount of water for extracting water-soluble protein was 80 mL,with the extraction rate of 37.13%,the optimal concentration of NaCl for extracting salt-soluble protein was 6% with the extraction rate of 25.34%,the optimal concentration of ethanol for extracting prolaminsoluble protein was 70% with the extraction rate of 3.8% and the optimal concentration of NaOH for extracting alkali-soluble protein was 0.3% while the extraction rate was 40.18%.In addition,the amino acid compositions of extracting protein and their analysis.

关 键 词：玉米胚芽 分离蛋白 提取率 氨基酸组分分析 

分 类 号：S513[农业科学—作物学]