pH吸附法纯化乳酸乳球菌素Lacticin LLC518  

The Studies on Purification of Lacticin LLC518 by pH-Adsorption Method

作　　者：方佳琪[1] 竺德强[1] 柯芳芳[1] 陈晓琳[1] 张明[1] 

机构地区：[1]安徽农业大学生命科学学院

出　　处：《食品与发酵工业》2011年第10期52-56,共5页Food and Fermentation Industries

基　　金：国家863计划项目(2008AA10Z319);安徽省高等学校省级质量工程项目(20101899)

摘　　要：对乳酸乳球菌素Lacticin LLC518在不同pH条件下产生菌的吸附作用进行研究,结果表明,pH 6.0时吸附率达100%,pH 2.0时吸附率为0。利用该特性,可将产生菌细胞从发酵液中分离,制备Lacticin LLC518粗制品。通过与硫酸铵沉淀样品进行对照,表明pH吸附法具有良好的分离效果,Lacticin LLC518的回收率为20%,纯化倍数高达83.02。The adsorption rates of Lacticin LLC518 on Lactococcus lactis subsp, lactis 518 at different pH conditions were studied. The results showed that the adsorption rate was 100% at pH 6.0 while no Laeticin LLC518 was adsorbed at pH 2.0. Therefore, the crude Lactiein LLC518 was prepared from the cell-free supernatant of the L. lactis subsp, lactis 518 culture when the exterior environment had a pH of 6.0 and 2.0 by absorbing and desorbing Laeticin LLC518, respeetively. The pH absorption method has an obvious advantage superior to the ammonium sulphate precipitation method for its better absorption effeet when the crude Laeticin LLC518 from precipitation with ammonium sulfate as a control. The speeifie activity of Laeticin LLC518 was increased 83.02 fold with a yield of 20%.

关 键 词：乳酸乳球菌素 pH吸附 分离 层析技术 效率 

分 类 号：TS201.3[轻工技术与工程—食品科学]