β-环糊精及其衍生物、金属离子协同增敏黄曲霉毒素B_1的荧光光谱分析及应用研究  

Studies and Application of Fluorescence of Aflatoxin B_1 Enhanced by Synergetic Effect of β-Cyclodextin and its Derivatives and Metalions

作　　者：张敏[1] 张宇昊[1,2] 马良[1,2] 

机构地区：[1]西南大学食品科学学院,重庆400716 [2]重庆市特色食品工程技术研究中心,重庆400716

出　　处：《分析化学》2011年第12期1907-1911,共5页Chinese Journal of Analytical Chemistry

基　　金：国家"863"计划(No.2007AA10Z427);中央高校基本科研业务费专项资金(No.XDJK2009C056);西南大学研究生科技创新基金项目(No.ky2010006)资助项目

摘　　要：采用荧光光度法研究了β-环糊精及其衍生物(β-CDs)-金属离子(M)体系对黄曲霉毒素B1(AFB1)的荧光增敏作用。在β-CD-Hg体系中,AFB1的荧光强度显著增强。当溶剂中甲醇比例为50%,Hg2+、β-CD与AFB1反应摩尔比均大于300∶1时,荧光增强倍数最大,可达到15倍。将β-CD-Hg作为新型荧光增强剂,替代国标方法中传统衍生试剂,建立了高灵敏度、快速测定AFB1的荧光分析法。AFB1浓度在0.1～40μg/L范围内与体系荧光强度呈线性关系,相关系数R为0.9998,检出限为0.08μg/L,回收率为90%～100%;与国标方法及速测方法比对分析,检测结果均无显著性差异。Fluorescence enhancement of AFB1 by system of β-CD-M was studied by fluorescence spectrophotometry.In system of β-CD-Hg,fluorescence intensity of AFB1 was markedly enhanced.When ratio of methanol in solvent was 50%,reaction molar ratio of β-CD and HgCl2 with AFB1 were both more than 300 ∶ 1,the fluorescence enhancement could achieve the maximum-16 times,significantly higher than various derivation reported and in National standard.β-CD-Hg was used as new fluorescence enhancement agent to replace traditional derivation,in order to improve the detection sensitivity.And a high sensitivity and rapid determination of AFB1 was established.The fluorescence intensity was linearly related to the AFB1 concentration in the range of 0.1-40 μg/L with a correlation coefficient of 0.9998.The detection limit was 0.08 μg/kg.The recovery was between 90-100% by spiking 10 μg/kg of AFB1 in samples.There are no significant differences between the new method and national standard method,as well as fast method.

关 键 词：Β-环糊精 黄曲霉毒素B1 荧光增强 三元络合物 

分 类 号：R114[医药卫生—卫生毒理学] O657.3[医药卫生—公共卫生与预防医学]