两步生物法转化柚皮苷制备L-鼠李糖  

Preparation of L-Rhamnose by Two-step Biotransformation of Naringin

作　　者：魏胜华[1] 孟娜[1] 李婉珍[1] 陶玉贵[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《精细化工》2011年第12期1178-1182,共5页Fine Chemicals

摘　　要：考察了利用柚苷酶和酵母静息细胞作为催化剂,两步生物法转化柚皮苷制备L-鼠李糖的工艺过程。柚苷酶水解柚皮苷的最佳工艺条件是ρ(柚皮苷)=14 g/L的水溶液为底物,加酶量10 mL/L(即每升底物中加入酶的体积,下同),pH=5.5,酶解时间22 h。酵母代谢葡萄糖的最佳工艺条件是酵母用量2.5 g/L(即每升水解液中加入酵母的质量,下同),温度32℃,pH=6.0,发酵时间120 min。在30 L发酵罐中进行了小试,最终获得了质量分数大于98.5%的鼠李糖结晶85.6 g,为理论得率的86.5%。The preparation of L-rhamnose by two-step biotransformation of naringin using naringinase and rest ceils of yeast as catalyst was studied in this paper. The optimum conditions of hydrolysis were as follows： the mass concentration of naringin at 14 g/L, 10 mL/L of naringinase, pH at 5.5, and reaction time of 22 h. The optimum conditions of fermentation were： the yeast dosage 2.5 g/L, the temperature at 32 ℃ ,pH at 6. 0, and fermentation time of 120 rain. A small-scale pilot was carried out in 30 L fermentor, and 85.6 g crystals of rhamnose was obtained, the purity of which was more than 98.5%. This yield accounted for 86. 5% of the theoretical yield.

关 键 词：柚皮苷 鼠李糖 柚苷酶 酵母 生物工程 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]