黄曲霉毒素B_1(AFB_1)降解菌的筛选鉴定  

Screening and Identification of Aflatoxin B_1(AFB_1) Degradation Strains

作　　者：陈晓飞[1] 周伏忠[1] 孙玉飞[1] 宁萌[1] 常丽[1] 

机构地区：[1]河南省科学院生物研究所河南省微生物工程重点实验室,郑州450008

出　　处：《河南科学》2011年第12期1447-1450,共4页Henan Science

摘　　要：以香豆素为唯一碳源和能源的培养基进行初筛,然后将菌株与黄曲霉毒素B1(AFB1)共培养后测定AFB1降解率的方法进行复筛,最后筛选出4株高活性AFB1降解菌;其中G3-21和T1-4-1对AFB1降解率可达到65%,Y2-30和T1-3-7对AFB1的降解率达到45%以上,形态和16S rDNA序列分析鉴定结果表明,G3-21和Y2-30为恶臭假单胞菌(Pseudomonas putida),T1-4-1为链霉菌(Streptomyces sp.),T1-3-7为厚壁菌(Firmicutes bacterium).Using cumarin plate as the priliminary screening procedure,then co-culture and AFB1 test as the second screening step of the strains. It was found that four strains of which have the AFB1 ability： the degradation ratio of AFB1 of G3-21 and T1-4-1 reached 65 %, the degradation ratio of other two strains Y2-30 and T1-3-7 reached 45 %. From the analysis of colony morphology and 16S sequence,the strains G3-21 and Y2-30 were finally identified as Pseudomonas putida. The strain Streptomyces sp. the strain T1-3-7was Firmicutes bacterium.

关 键 词：黄曲霉毒素B1 降解菌 筛选 鉴定 

分 类 号：TS201.6[轻工技术与工程—食品科学]