养殖水产品中孔雀石绿、结晶紫及其代谢产物的前处理方法研究  

Study on the Pre-treatment Methods for Detection of Malachite Green,Crystal Violet and Their Metabolites in Farmed Aquatic Products

作　　者：郑跃进 李昊[2,3] 林青 刘帅[2] 高小尧[2] 沈小妙[2] 饶平凡[2] 

机构地区：[1]福州出入境检验检疫局,福州350001 [2]福州大学生物科学与工程学院,福州350108 [3]福州蓝昊生物医药有限公司,福州350002 [4]国家加工食品质量监督检验中心(福州),福州350002

出　　处：《中国食品学报》2011年第7期200-207,共8页Journal of Chinese Institute Of Food Science and Technology

摘　　要：根据SN/T1768-2006、GB/T19857-2005、GB/T20361-2006标准,比较适合以上标准的3种水产品前处理方法,即:固相萃取法、旋转蒸发法、氮气吹干法。采用液相色谱串联质谱(LC-MS)检测水产品中孔雀石绿(MG)、结晶紫(CV)含量。以3种前处理方法所得回收率为指标,确定固相萃取法为适合的方法。换用不同的固相萃取小柱,对固相萃取法进行优化,以获得最大回收率。结果表明固相萃取小柱LH的最佳回收率超过88%,该固相小柱的重复使用回收率损失仅3%。该方法简便、快速、灵敏,适于水产品中孔雀石绿及其代谢物隐色孔雀石绿,结晶紫及其代谢物隐色结晶紫残留量的检测。According to SN/T1768-2006,GB/T19857-2005,GB/T20361-2006 standard,to compare three different pre-treatment methods（solid phase extraction,rotary evaporation and nitrogen blow dry method） of aquatic products.Use Liquid chromatography tandem mass spectrometry to measuring contents of MG and CV in the sample.Change different solid-phase extraction column in pre-treatment to find the best column.The result showed that Solid Phase Extraction is the best determination way with higher recovery rate over 88%.Conclusion：This pre-treatment method with LH solidphase column is simple,fast and sensitive,which is benefit for the detection of malachite green as well as metabolite leuco malachite green,gentian violet and crystal violet leuco metabolites residues.

关 键 词：固相萃取法 液相色谱串联质谱 孔雀石绿 隐性孔雀石绿 结晶紫 隐性结晶紫 水产品检测 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]