纤维素酶制备壳寡糖工艺条件的研究  

Study on the Process Condition of Chito-oligosaccharide Preparation by Cellulose Enzyme Degradation

作　　者：谭佩毅[1] 黄秀锦[1] 杨福臣[1] 袁书林[1,2] 郭小华[2] 

机构地区：[1]江苏食品职业技术学院,江苏淮安223003 [2]淮安淮大科技发展有限公司,江苏淮安223005

出　　处：《农业科技与装备》2011年第10期60-62,64,共4页Agricultural Science & Technology and Equipment

基　　金：淮安市科技支撑计划(农业)项目(SN0876);江苏省科技型企业创新资金(BC2010142)

摘　　要：研究纤维素酶降解壳聚糖生产壳寡糖的工艺条件,对影响壳聚糖降解的因素进行探讨,通过正交试验优化酶解反应工艺参数。结果表明:影响壳聚糖降解的主次顺序分别为反应时间>纤维素酶和壳聚糖比例>温度>pH值;最佳酶解条件为温度40℃,纤维素酶∶壳聚糖=1∶6,pH值5.0,反应时间8h。壳寡糖的得率为96.5%。The production process of chito-oligosaccharide by cellulose enzyme degradation were researched. The influence racquet＇s ,of chito-oligosaccharide degradation were discussed. And the cellulose enzyme reaction process parameter was optimized tt,rough orthogonal experiment in this paper. The results showed that the sequence of influencing chito-oligosaceharide degradation was the reaction time〉 cellulose enzyme and chito-oligosaccharide proportion〉temperature〉pH. The best zymolysing conditions were temperature 40℃,cellulose enzyme： chito-oligosaccharide for 6, pH 5, reaction time 8 h. The chito-oligosaccharide output was 96.5% under the condition of optimization degradation conditions.

关 键 词：纤维素酶 壳寡糖 降解 工艺 

分 类 号：TS245.9[轻工技术与工程—制糖工程]