不透明红球菌转化合成α-酮异己酸培养基优化  

Medium optimization for biosynthesis of α-ketoisocaproate by Rhodococcus opacus DSM 43250

作　　者：祝玉洪[1] 刘龙[1] 周景文[1] 房峻[1] 李江华[1] 堵国成[1] 陈坚[2] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122 [2]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《生物加工过程》2011年第6期6-10,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB714306);国家重点基础研究发展计划(973计划)前期研究专项资助项目(2010CB535014);国家杰出青年基金资助项目(20625619);国家自然科学基金重点资助项目(20836003);中央高校基本科研业务费专项资助项目(JUSRP30901)

摘　　要：利用基于统计学的方法对不透明红球菌(Rhodococcus opacus)DSM 43250转化合成α-酮异己酸(KIC)的培养环境进行优化。采用Plackett-Burman(PB)设计筛选获得对KIC产量具有显著影响的关键营养因子:(NH4)2SO4、麦芽膏和NaNO3。通过最陡爬坡实验、Box-Behnken实验设计和SAS软件回归分析建立了KIC产量关于3个关键营养因子的二次多项式模型,并以模型求解确定最佳培养条件(g/L):(NH4)2SO4 0.23,麦芽膏2.42,NaNO3 1.43。在此培养条件下,KIC的理论最高产量为23.98 mg/L,在验证实验中KIC最高产量为23.93 mg/L,比优化前(3.72 mg/L)提高了6.43倍。The culture medium for biotransformation of α-ketoisocaproate（KIC） by Rhodococcus opacus DSM 43250 was optimized by a statistical analysis based approach.Firstly,Plackett-Burman design was adopted for the screening of key nutrient factors influencing α-ketoisocaproate transformation,and（NH4）2SO4,malt extract,and NaNO3 were determined to be three key factors.Then,the steepest ascent experiments were conducted to reach the optimal response area.Finally,a second-order polynomial model was constructed by Box-Behnken design and the regression analysis was carried out by SAS software.The optimal culture medium compostion was：（NH4）2SO4 0.23 g/L,malt extract 2.42 g/L,and NaNO3 1.43 g/L.With the obtained culture medium,the maximal KIC production was predicted to be 23.98 mg/L,and in the validation experiments,KIC production reached 23.93 mg/L,which was 6.43-fold of that of control without optimization（3.72 mg/L）.

关 键 词：α-酮异己酸 优化 不透明红球菌 统计学分析 

分 类 号：Q939.97[生物学—微生物学]