微生物资源库中产纤维素酶菌株的筛选及其酶学性质研究  

Screening and identification of cellulase-secreting strains from CICIM-CU and their enzymatic properties

作　　者：陈源源[1,2] 沈微[1,2] 石贵阳[1,2] 王正祥[1,2] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122 [2]江南大学中国高校工业微生物资源与信息中心,无锡214122

出　　处：《工业微生物》2011年第6期15-19,共5页Industrial Microbiology

基　　金：国家自然资源科技平台(No.2005DKA21208);教育部科技基础条件平台(No.505006)资助

摘　　要：利用平板碘液染色法对中国高校工业微生物资源与信息中心(CICIM-CU)资源库中保藏的625株细菌和放线菌进行筛选,从中筛出112株具有纤维素酶产生能力的菌株,建立了一个产纤维素酶微生物资源库。对筛选得到的纤维素酶产生菌株进行16S rDNA鉴定后发现,其中的Streptomyces tanashiensis、Paenibacillus tundrae和Comamonas testosteroni等微生物产生的纤维素酶国内外未见文献报道。对Streptomyces tanashiensis CICIM B4515菌株产生的纤维素酶的酶学性质进行了初步研究,发现其最适反应温度为50℃,在60℃以下活性稳定;最适反应pH为6.0,在pH 5.0～7.0范围内酶活相对稳定。较好的温度耐受性和较宽的pH作用范围使得该菌株产生的纤维素酶具有一定的工业应用前景。112 strains producing cellulase were screened from 625 bacterial and actinomycetes isolates conserved in culture and information center of industrial microorganism of China universities （CICIM-CU） by iodine coloration method. These strains were identified based on 16S rDNA sequence analysis. The results showed that three newly isolated producers of cellulase, including Comamonas testosterone, Paenibacillus tundrae and Streptomyces tanashiensis were screened. For the first time, cellulase produced by Streptomyces tanashiensis was reported in this study. The optimal conditions for the enzymatic reaction were about 50 ℃ and pH 6.0. At pH 5.0-- 7.0, the enzyme showed high activity and good stability. This new thermotolerable and pH stable cellulase may make a significant contribution to the cellulose related industry.

关 键 词：纤维素酶 筛选 田无链霉菌 酶学性质 

分 类 号：TQ925[轻工技术与工程—发酵工程]