饲料中添加芽孢杆菌对草鱼表观消化率及消化酶活性的影响  

Dietary supplement of Bacillus spp.affected apparent digestibility,digestive enzyme activity of Ctenopharyngodon idellus

作　　者：曲艺[1] 吴志新[1] 杨丽[1] 彭小云[1] 袁娟[1] 毕鹏[1] 刘红[1] 陈孝煊[1] 

机构地区：[1]华中农业大学水产学院,武汉430070

出　　处：《华中农业大学学报》2012年第1期106-111,共6页Journal of Huazhong Agricultural University

基　　金：国家科技支撑计划项目(2007BAD37B02);湖北省"十一五"科技攻关项目(2006AA203A02;2007AA203A01)

摘　　要：在草鱼(Ctenopharyngodon idellus)饲料中分别添加0(Ⅰ组)、1×108(Ⅱ组)、3×108(Ⅲ组)、5×108cfu/g(Ⅳ组)芽孢杆菌(Bacillus spp.)持续投喂草鱼,收集粪便测定草鱼对各营养物质的表观消化率和消化酶活性。结果表明:投喂30d后,Ⅱ、Ⅲ组干物质、粗蛋白、粗脂肪表观消化率和Ⅳ组粗蛋白、粗脂肪表观消化率均显著高于Ⅰ组(对照组),其中Ⅱ组的表观消化率最高,且芽孢杆菌添加量与营养物质表观消化率最符合一元二次曲线方程。Ⅱ组中肠和Ⅱ、Ⅲ组肝胰脏蛋白酶活性均显著提高;Ⅱ组的前肠和后肠淀粉酶活性显著高于对照组;Ⅱ、Ⅲ组后肠脂肪酶活性均显著高于对照组。投喂60d后,消化酶活性也有相似的结果。双因子方差分析表明:芽孢杆菌的添加量对中肠、后肠和肝胰脏蛋白酶活性,前肠、中肠和后肠淀粉酶活性,中肠和后肠脂肪酶活性均有显著影响;投喂时间对肝胰脏蛋白酶、淀粉酶活性有显著影响。Ctenopharyngodon idellus were fed with basal diet supplemented with 0（Ⅰ）、1×10^8（Ⅱ）、3×10^8（Ⅲ）、5×10^8cfu/g（Ⅳ） Bacillus spp. respectively for 30 days. Feces was collected to detect apparent digestibility of feed nutrients. The apparent digestibility of dry matter, crude protein and crude lipid in I group was significantly lower than the other treatment groups （P〈0. 05）. Quadratic curve e- quation was the best to fit the relationship between dosages of Bacillus spp. and apparent digestibility of nutrients. Protease activity of the mid-gut in Ⅱ group and the hepatopancreas in Ⅱ, Ⅲgroups increased significantly compared with I group （P〈0. 05）. Amylase activity of the fore-gut and hind-gut in Ⅱgroup increased significantly compared with I group （P〈0. 05）. Lipase activity of the hind-gut in Ⅱ, Ⅲ group increased significantly compared with I group （P〈0. 05）. After feeding for 60 days,the similar results appeared. Two-way analysis of variance showed that protease activity of the mid-gut, hind-gut and the hepatopancreas, amylase activity of the fore-gut, mid-gut and hind-gut, lipase activity of the mid- gut and hind-gut were all significantly affected by the additional doseages of Bacillus spp. （P〈0. 05）. Protease activity and amylase activity of the hepatopancreas were significantly affected by feeding days （P〈0. 05）.

关 键 词：草鱼 芽孢杆菌 表观消化率 蛋白酶活性 淀粉酶活性 脂肪酶活性 

分 类 号：S963.739[农业科学—水产养殖] Q959.468[农业科学—水产科学]