血吸虫血红蛋白降解途径的重构及关键酶蛋白保守结构域的分析  

Construction of Hemoglobin Degradation Pathway of Schistosoma and Analysis of Conserved Domains of Pathway Key Enzymes

作　　者：魏佳[1,2] 李园园[2] 李亦学[2] 于复东[2] 

机构地区：[1]华东理工大学生物工程学院,上海200237 [2]上海生物信息技术研究中心,上海200235

出　　处：《华东理工大学学报（自然科学版）》2011年第6期706-714,共9页Journal of East China University of Science and Technology

基　　金：国家自然科学基金(30900834)

摘　　要：利用生物信息学方法,以疟原虫的血红蛋白降解途径为基础,成功地构建出了日本血吸虫和曼氏血吸虫的血红蛋白降解途径,并对降解途径上的关键酶蛋白的保守结构域进行了序列及结构分析。预测得到的血吸虫血红蛋白降解酶保守结构域,与疟原虫相比,在序列上催化位点高度保守,在结构上空间相对位置较一致,为研究血吸虫血红蛋白降解酶,及其与底物或抑制剂在三维结构上的相互作用提供了理论基础。On the basis of hemoglobin degradation pathway of Plasmodium falciparum, hemoglobin degradation pathways of Schistosoma japonicum and Schistosoma mansoni were constructed using bioinformatic methods. Sequences and structures of conserved domains of pathway key enzymes were analyzed after pathway construction. The catalytic sites of predicted hemoglobinases of Schistosoma are highly conserved with Plasmodium in both sequence and structure level. This paper provides a theoretical foundation for Schistosoma hemoglobinase study, and analysis of their interactions with substrates or inhibitors in three-dimensional level.

关 键 词：日本血吸虫 曼氏血吸虫 疟原虫 血红蛋白降解途径 保守结构域 

分 类 号：Q811.4[生物学—生物工程]