HPLC法测定大豆磷脂中卵磷脂含量  

Quantitative Analysis of Lecithin in Phospholipids by HPLC

作　　者：何新霞[1] 郑孝华[1] 郏海燕 叶锋 

机构地区：[1]浙江师范大学生物系,金华321004

出　　处：《食品科学》2000年第2期57-59,共3页Food Science

摘　　要：应用Lichrosorb si６０（５ m）２５０ ４mm。色谱柱，乙腈：甲醇：水（６５：２１：１４Ｖ／Ｖ）ｈ流动相外标法测定了大豆磷脂中卵磷脂含量、卵磷脂检测线性范围为０.０４－０.８mg／ml。r=０.９９９６，方法的回收率为９８．３５％，ＲＳＤ为６.９４% (n＝６）。A simple rapid and accurate quantitative method was established for the determination of lecithin in soybean phospholipids by HPLC.Separation was obtained by using Lichrosorb si60 column (5 m250 x 4mm ) and mobile phase of acetonitrile:methanol:water (65:21:14v/v) with flow rate of 1.8ml/min .The eluates were monitored with UV detector at 205nm.The retention time for lecithin was 4.875min .The lecithin curve was linear in the range of 0.04 0.08mg/ml with =0.9996.The average recovery rate for lecithin was 98.35%.RSD=6.94%(n=6).

关 键 词：大豆磷脂 卵磷脂 高效液相色谱法 含量测定 

分 类 号：Q545.13[生物学—生物化学] TS214.1[轻工技术与工程—粮食、油脂及植物蛋白工程]