复合诱变选育表面活性素(surfactin)高产菌株  

Breeding of High-yield Surfactin-producing Strain by a Combined Mutagenesis Approach

作　　者：郭芳芳[1] 李金良[1] 陆兆新[1] 别小妹[1] 张充[1] 吕凤霞[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品科学》2011年第23期270-276,共7页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD23B05);国家自然科学基金项目(30871753)

摘　　要：为筛选抗菌脂肽表面活性素高产菌株,以枯草芽孢杆菌Bacillus subtilis fmbJ224为出发菌株,先后采用氯化锂(LiCl)、亚硝基胍(NTG)、甲基磺酸乙酯(EMS)和链霉素(streptomycin)进行复合诱变。结果表明:经初筛、复筛选育得到1株高产抗菌脂肽菌株LN2-3,其Surfactin产量达到239.2mg/L,比出发菌株Bacillus subtilis fmbJ224(5.25mg/L)提高了44.56倍,且该菌株具有良好的遗传稳定性。提示采用LiCl-NTG复合诱变能较大幅度的提高诱变率,可以获得抗菌脂肽高产菌株。In order to breed a high-yield surfactin-producing strain,Bacillus subtilis fmbJ224 was used as the start strain and mutagenized sequentially with lithium chloride（LiCl）,nitrosoguanidine（NTG）,ethyl methane sulphonate（EMS） and streptomycin.The preliminary and secondary screening results showed that strain LN2-3 had high ability to produce surfactin,with a yield of 239.2 mg/L,which revealed a 44.56-fold increase compared with the original strain.Meanwhile,the strain had good genetic stability.This study demonstrates that the mutation rate of the original strain could be raised greatly by the combined use of LiCl and NTG.

关 键 词：芽孢杆菌 亚硝基胍(NTG) LICL 复合诱变 表面活性素 

分 类 号：TS201.3[轻工技术与工程—食品科学]