高效离子色谱法测定食品中低聚果糖的含量  

Determination of Fructooligosaccharide in Foods by High Performance Ion Exchange Chromatography Method

作　　者：张绩觅[1] 刘玉峰[1] 唐华澄[1] 李东[1] 

机构地区：[1]北京市营养源研究所分析室,北京100069

出　　处：《食品研究与开发》2012年第1期135-138,共4页Food Research and Development

摘　　要：建立离子色谱法定量测定食品中低聚果糖的方法,应用CarboPacTMPA1(4 mm×250 mm)阴离子交换柱,氢氧化钠和醋酸钠淋洗液梯度淋洗,脉冲安培检测器检测。6次测定低聚果糖口服液,相对标准偏差为2.31%～5.14%,3种糖的加标回收率在96.23%～105.03%之间,本方法简单快捷,适用于食品中低聚果糖的检测。A method was established to determine FOS in foods by high performance ion exchange chromatography method with pulsed amperometric detection. Methods In the present study, the gradient procedure was used to separate the mixed standard on a CarboPac PAl （4 mm×250 mm） anion exchange column. The repeatability relative standard deviations of this method was 2.31%-5.14 % and the recovery was 96.23 %-105.03 %. The method was fit for determining FOS in food.

关 键 词：高效离子色谱法 脉冲安培检测 低聚果糖 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.75[轻工技术与工程—食品科学与工程]