利用大孔吸附树脂吸附分离藏红花细胞培养液中藏红花素和藏红花苦素  

Separation of Crocin and Picrocrocin from Saffron Cell Culture Broth by Macroporous Resin Adsorption

作　　者：吴频梅[1] 袁丽红[1] 黄晶[1] 孙镇[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《食品科学》2011年第24期1-6,共6页Food Science

基　　金：国家"973"项目(2009CB724700)

摘　　要：目的:研究大孔树脂提取藏红花细胞培养液中藏红花素和藏红花苦素的工艺。方法:对4种大孔树脂提取藏红花素和藏红花苦素的效果进行比较,考察HPD-100A大孔树脂提取藏红花素和藏红花苦素的最佳工艺条件。结果:HPD-100A树脂提取藏红花素和藏红花苦素效果最佳,其最适工艺条件为25℃、色素液pH6、藏红花素和藏红花苦素上样质量浓度分别为1.0mg/mL和24.6mg/mL、溶液处理量1.5BV、吸附流速1.5mL/min、洗脱剂为体积分数40%乙醇溶液、洗脱剂体积1.7BV、洗脱流速1.0mL/min。藏红花素和藏红花苦素的吸附率分别达到94.4%和75.5%,解吸率分别为99.9%和87.5%。结论:采用大孔吸附树脂吸附分离藏红花培养液中藏红花素和藏红花苦素方法可行,前景广阔。Objective： The separation of crocin and picrocrocin from saffron cell culture broth was investigated by macroporous resin adsorption.Methods： The effectiveness of 4 types of macroporous resin was compared in separating crocin and picrocrocin.The application of HPD-100A resin for separating crocin and picrocrocin from saffron cell culture broth was optimized.Results： HPD-100A resin was the best resin to separate crocin and picrocrocin from saffron cell culture broth among the investigated ones.The best separation of crocin and picrocrocin was achieved as an adsorption rate of 94.4% and 75.5% and a desorption rate of 99.9% and 87.5%,respectively when the cell culture broth was concentrated until a concentration of 1.0 mg/mL and 24.6 mg/mL for crocin and picrocrocin,respectively,temperature 25 ℃,pH 6,and 1.5 BV of each concentrate was loaded onto the column at a flow rate of 1.5 mL/min after being adjusted to pH 6 and then eluted with 1.7 BV of 40% aqueous ethanol at a flow rate of 1.0 mL/min.Conclusion： Macroporous resin adsorption is feasible and holds promise for separating crocin and picrocrocin from saffron cell culture broth.

关 键 词：大孔吸附树脂 藏红花素 藏红花苦素 提取 藏红花细胞培养 

分 类 号：TS202.3[轻工技术与工程—食品科学]