茜草总黄酮提取工艺研究  

Optimization of Extraction Process for Total Flavonoids from Madder Root

作　　者：田春莲[1] 蒋凤开[2] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000 [2]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《食品科学》2011年第24期60-63,共4页Food Science

基　　金：湖南省科技计划项目(2010TP4007-1);林产化工工程湖南省重点实验室开放基金项目(JDZ200906);湖南省高校"林产资源化学与林化产品开发"科技创新团队支持计划项目(湘教通(2010)212号)

摘　　要：研究茜草总黄酮的提取工艺条件。采用热浸提法提取茜草总黄酮,硝酸铝显色法测定总黄酮提取率。通过单因素试验研究乙醇体积分数、料液比、提取温度、提取时间对黄酮提取率的影响,通过正交试验进一步优化提取工艺条件。单因素试验结果显示,乙醇体积分数50%、提取温度70℃、提取时间2.5h、料液比1:40(g/mL)条件下茜草总黄酮提取率较高;正交试验结果表明:茜草总黄酮的最佳提取工艺为60%、乙醇溶液提取2h、提取温度70℃、料液比1:40(g/mL),此条件下茜草总黄酮提取率为1.67%。本工艺简单可行,有较好的参考价值。This study was carried out to optimize the hot water extraction of total flavonoids from madder root.The effects of 4 process parameters including ethanol concentration,material-to-liquid ratio,temperature and extraction duration on extraction efficiencywere investigated by one-factor-at-a-time method and orthogonal array design.The results form the one-factor-at-a-time experiments showed that the optimal extraction conditions were 50% aqueous ethanol as extraction solvent at a material-to-liquid ratio of 1：40（g/mL）,temperature 70℃ and extraction duration 2.5 h.The results from the subsequent orthogonal array design experiments indicated that the highest extraction efficiency（1.67%） for madder root was achieved after 2 h of extraction with 60% aqueous ethanol at 70 ℃ and a material-to-liquid ratio of 1：40（g/mL）.In conclusion,the optimized extraction process is simple and feasible and has great reference value.

关 键 词：茜草 总黄酮 提取工艺 

分 类 号：TS272[农业科学—茶叶生产加工]