超声提取羊胎盘水溶性活性蛋白  

Study on Ultrasonic Extraction of Activity Protein from Sheep Placenta

作　　者：焦艳丽[1] 杜冰[1] 郭晓明[1] 倪婉娜[1] 李明玉[1] 杨公明[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《食品与发酵工业》2011年第11期224-228,共5页Food and Fermentation Industries

基　　金：广东省科技型中小企业技术创新专项资金大学生创业项目(2009CD030)

摘　　要：以淋巴细胞增殖率为主要指标,在单因素的基础上,采用响应面法优化了超声提取羊胎盘中水溶性活性蛋白质的工艺,建立了二次多项模型,研究超声功率、固液比、超声时间3个因素及其交互作用对提取工艺的影响。试验结果表明,对蛋白质提取率的影响次序为超声功率>固液比>超声时间;最优工艺参数是:超声功率为300W,固液比为1:30,超声时间25min,淋巴细胞增殖率达53.17%。In order to timely extract protein compounds from Sheep Placenta,investigation of ultrasonic -assisted extraction was carried.The entire experiment was carefully designed and optimized with response surface method （RSM）.A second quadratic equation for protein extraction was finally obtained.Factors,such as extraction temperature, solid-liquid ratio extraction time and their corresponding relationships were investigated.Results showed that the effectiveness order of four factors is as follows：solid-liquid ratio〉 extraction time 〉extraction temperature.The optimized extraction conditions were solid-liquid ratio〉 extraction time 〉extraction temperature.Under the optimal conditions,the extraction rate of protein from Sheep Placenta reached to 53.17%.

关 键 词：羊胎盘 水溶性蛋白质 响应面法 

分 类 号：R284.1[医药卫生—中药学]