LHJ增溶剂对难溶性食品中添加剂定量测定方法的研究  

The research on LHJ solubilizer use in quantitative determination method of additives in water-insoluble food

作　　者：李建英[1] 高海红[1] 高学英[1] 邢沛然[1] 邢玉伟[1] 韩永红[1] 郝宁[1] 

机构地区：[1]河北省邯郸市疾病预防控制中心,河北邯郸056008

出　　处：《中国卫生检验杂志》2011年第12期2828-2829,2832,共3页Chinese Journal of Health Laboratory Technology

基　　金：邯郸市科学技术研究发展计划项目(0823108056-4)

摘　　要：目的:建立难溶性食品中添加剂定量测定方法。方法:样品加入新型LHJ增溶剂,使其中的食品添加剂充分溶解,加入亚铁氰化钾、乙酸锌沉淀蛋白,定容,离心,取上清液过0.45μm滤膜,取10μl进高效液相色谱仪测定。采用C18色谱柱,以甲醇+乙酸铵(5+95)为流动相,流速1.0 ml/min,柱温30℃,波长230 nm紫外检测器,根据保留时间定性,外标峰面积定量。结果:该方法在0.010 mg/ml～0.100 mg/ml范围内线性关系良好,相关系数均大于0.9999,检出限为0.00015 g/kg回收率在90%～108%之间。结论:该方法简便,快捷,回收率高,精密度好,处理样品时使用新型LHJ增溶剂测定结果提高2倍～3倍。Objective：To establish a method for quantitative determination of additives in insoluble food.Methods： The new LHJ solubilizer was added into samples to make the food additives fully dissolved.Then potassium ferrocyanide and zinc acetate were added to precipitate protein.After constant volume and centrifugation,the supernatant was filtered over 0.45 μm membrane,then 10 μl filtrate was taken for determination by high performance liquid chromatography.C18 column was used with methanol and ammonium acetate（5＋95） as the mobile phase,flow rate of 1.0 ml/min,column temperature at 30℃ and UV detector wavelength at 230 nm in this method,then qualitative and quantitative analysis were conducted according to retention time and peak area respectively.Results： Several additives in 0.010 mg/ml^0.100 mg/ml are in a good linear relationship,the correlation coefficients are greater than 0.9999,the detection limit is 0.00015 g/kg,the recovery rate is in the range of 90%~108%.Conclusion： The method is simple and rapid with high recovery rate and good precision.Using the novel LHJ solubilizer in sample preparation can improve efficiency of results by 2~3 times.

关 键 词：食品添加剂 LHJ增溶剂 难溶性食品 高效液相色谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]