流动注射-化学发光法快速测定糖精钠  

Rapid Determination of Sodium Saccharin by FI-Chemiluminescence

作　　者：高向阳[1] 魏姜勉[1] 王珊[1] 尹中瑞[1] 

机构地区：[1]河南农业大学食品科学技术学院,郑州450002

出　　处：《理化检验（化学分册）》2011年第12期1446-1449,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省重点学科建设基金资助(10466-X-082301)

摘　　要：提出了基于鲁米诺与过氧化氢化学发光反应的流动注射-化学发光法快速测定糖精钠的方法。在酸性介质中,糖精钠与重铬酸钾反应定量生成铬(Ⅲ),所得的铬(Ⅲ)可显著增加上述化学发光反应的化学发光强度,且其强度的增加值与糖精钠浓度在5.0×10-7～1.0×10-5 mol.L-1范围内呈线性关系,方法的检出限(3s)为1.08×10-11 mol.L-1。在试样溶液中分别加入3个浓度水平的糖精钠标准溶液,按方法测定求得其回收率在95.6%～105.8%之间,相对标准偏差(n=12)为2.9%。FI-Chemiluminescence determination of sodium saccharin based on the CL reaction between luminol and H2O2 was reported.In an acid medium,the solution of sodium saccharin was reacted with K2Cr2O7 to reduce Cr（Ⅵ） quantitatively to Cr（Ⅲ） and the CL intensity of the reaction system was remarkably enhanced by the Cr（Ⅲ） formed.The content of Cr（Ⅲ） was then determined and converted into contents of sodium saccharin.Linear relationship between values of increase in CL intensity and concentration of sodium saccharin was obtained in the range of 5.0×10-7-1.0×10-5mol·L-1.Detection limit（3s） was found to be 1.08×10-11mol·L-1.Recovery was tested by addition of standard solutions at 3 concentration levels to sample solutions,values of recovery obtained were in the range of 95.6%-105.8% with RSD（n=12） of 2.9%.

关 键 词：流动注射-化学发光法 食品添加剂 糖精钠 

分 类 号：O657.39[理学—分析化学]