胸腔积液细胞PTEN基因突变与肺癌相关性的探讨  

Study of the Mutation of PTEN Gene in Pleural Effusion Cells of Lung Cancer Patients

作　　者：车德海[1] 张华[1] 魏海波[1] 于雁[1] 

机构地区：[1]哈尔滨医科大学附属三院肿瘤内科,哈尔滨150081

出　　处：《现代检验医学杂志》2011年第6期49-51,共3页Journal of Modern Laboratory Medicine

基　　金：黑龙江省卫生厅科研项目：课题编号：2007-373.

摘　　要：目的 研究胸腔积液细胞抑癌基因PTEN（phosphatase and tension homology deleted on chromosome ten,PTEN） 基因突变高发区外显子5,7,8突变率与肺癌临床分期、病理组织学分级和病理类型的相关性.方法 研究组为52例伴有胸腔积液的肺癌患者,对照组为40例伴有胸腔积液的结核性胸膜炎和其他胸膜炎患者,采用聚合酶链反应-单链构象多态性分析（PCR-SSCP）法,对胸腔积液细胞PTEN基因外显子5,7,8进行突变分析.结果 52例肺癌胸腔积液细胞中检测到PTEN基因突变7例,均为外显子5突变,突变率为13.5%（7/52）;40例良性胸腔积液中未检测到PTEN基因突变,二者差异有统计学意义（P〈0.05）.52例肺癌患者不同临床分期中,Ⅲ期突变率为3.4%（1/29）,Ⅳ期突变率为26%（6/23）,二者差异有统计学意义（P〈0.05）;不同病理组织学分级中,低分化癌突变率为31%（5/16）,中、高分化癌突变率为5.6%（2/36）,二者差异有统计学意义（P〈0.05）;不同病理分型中,腺癌突变率为10.7%（3/28）、鳞癌突变率为14%（2/14）、小细胞肺癌突变率为20%（2/10）,三者差异无统计学意义（P〉0.05）.结论 肺癌胸腔积液细胞中存在PTEN基因突变,临床分期越晚、病理组织学分级越低,PTEN基因突变率越高,但与肺癌病理类型无关.PTEN基因突变可以作为有价值的肿瘤标记物用于恶性胸腔积液的诊断,可作为判断肺癌预后的一项指标.Objective To study the mutation frequencies of the exon5 exon7 and the exon8 of PTEN （phosphatase and tension in homology deleted on chromosome ten） gene in pleural effusion cells of lung cancer patients and investigate the relationship of the gene mutation and clinical stage and pathological differentiation. Methods 52 pleural effusion cells of lung cancer patients and 40 pleural effusion cells of benign lung disease patients were analyzed to detect the mutation of the exon5 exon7 and the exon8 of PTEN with PCR-SSCP. Results 13.5% （7/52） of lung cancer patients were detected to show PTEN gene mutations in the pleural effusion cells. The seven patients were the mutation of exon 5. No PTEN gene mutation was found in pleural effusion cells of benign lung disease patients（ P〈0. 05）. The mutation rates of PTEN gene in clinical stage Ⅲ 3.4%（1/29） had significant difference with that in clinical stage Ⅳ 26%（6/23） （ P〉0. 05）. The mutation rates of PTEN gene in poorly differentiated and well differentiated samples were 31% （5/16） and 5.6% （2/36） respectively （P〈0.05）. There was no significant difference between adenocarcinoma groups 10. 7% （3/28）, squamous cell carcinoma 14 % （2/14）and small cell carcinoma 20% （2/10）, （PD 0. 05）. Conclusion PTEN gene mutation could be seen in pleural effusion cells of lung cancer patients. There was an increasing tendency in rate of PTEN gene mutation in advanced and poorly differentiated lung cancer, and the mutation rate is not related to pathological types. The mutation of PTEN gene may be a valuable tumor marker in the differential diagnosis of malignant pleural effusion and prognosis of lung cancer.

关 键 词：肺癌 胸腔积液 PTEN基因 突变 

分 类 号：Q754[生物学—分子生物学] R734.2[医药卫生—肿瘤]