N~＋离子注入-紫外复合诱变选育纤维素酶高产菌株  

Screening of strain producing high-yield cellulase through composite mutagenesis by N^+ implantation and ultraviolet

作　　者：陈阿娜[1] 汤斌[1] 刘标[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽芜湖241000

出　　处：《食品工业科技》2012年第3期142-144,213,共4页Science and Technology of Food Industry

基　　金：安徽工程大学青年科研基金项目(2007YQ001zd);安徽省高校自然科学基金重点资助项目(KJ2007A018)

摘　　要：对产纤维素酶菌株Rhizopus sp.TY1原生质体进行N+离子注入-紫外复合诱变,以期获得高产菌株。以原生质体形成率和再生率为指标,确定最佳酶解时间3h;以致死率和正突变率为指标,确定最佳N+离子注入剂量为2.0×1015ions/cm2、最佳紫外照射时间为90s。实验筛选得到一株高产菌株Rhizopus sp.TY1.2,液态发酵终点时滤纸酶活力(FPA)和羧甲基纤维素酶活力(CMC)分别达到5.1、20.9U/mL,与出发菌株相比,FPA和CMC分别提高了75.9%和175.0%。传代实验结果显示Rhizopus sp.TY1.2产纤维素酶性能稳定,该结果表明N+离子注入-紫外复合诱变所产生的变异是可遗传的变异。Protoplast of Rhizopus sp.TY1 producing cellulase was mutagenized by N＋ implantation and ultraviolet composite mutagenesis to screen of strain producing high-yield cellulase.The best enzymolysis time was 3h with the formation rate and regeneration rate of the protoplast as selection criteria.The best dose of N＋ implantation was 2.0×1015ions/cm2 and the best irradiation time of ultraviolet was 90s with the mortality rate and positive mutation rate as selection criteria.A strain producing high-yield cellulase Rhizopus sp.TY1.2 was screened,which the FPA and CMC was 5.1U/mL and 20.9U/mL respectively at the end of liquid fermentation.The FPA and CMC were increased by 75.9% and 175.0% respectively compared to that of Rhizopus sp.TY1.Ten times subculturing showed that the mutant had good genetic stability and character of cellulase production.The results indicated that the mutation of Rhizopus sp.TY1.2 was descendible.

关 键 词：纤维素酶 原生质体 N+离子注入 紫外 复合诱变 

分 类 号：TS201.25[轻工技术与工程—食品科学]