Two genes, rill5 and rill6, of the rifamycin biosynthetic gene cluster in Amycolatopsis mediterranei likely encode a transketolase and a P450 monooxygenase, respectively, both essential for the conversion of rifamycin SV into B  

作　　者：Hua Yuan Wei Zhao Yi Zhong Jin Wang Zhongjun Qin Xiaoming Ding Guo-Ping Zhao 

机构地区：[1]Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China [2]State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai 200433, China [3]Department of Microbiology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China [4]Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai and National Engineering Center for BioChip at Shanghai, Shanghai 201203, China

出　　处：《Acta Biochimica et Biophysica Sinica》2011年第12期948-956,共9页生物化学与生物物理学报（英文版）

摘　　要：Amycolatopsis mediterranei produces an important anti- biotic rifamycin, the biosynthesis of which involves many unusual modifications. Previous work suggested a puta- tive P450 enzyme encoded by rill6 within the rifamycin biosynthetic gene cluster （rif） was required for the conver- sion of the intermediate rifamycin SV into the end product rifamycin B. In this study, we genetically proved that a putative transketolase encoded by rill5 is another essential enzyme for this conversion. Expression of merely rill5 and rifl6 in a r/f cluster null mutant ofA. mediterranei U32 was able to convert rifamycin SV into B. However, this Rifl5- and Rifl6-mediated conversion was only detected in intact cells of A. meidterranei, but not in Streptomyce coelicolor or Mycobacterium smegmatis, suggesting that yet-characterized gene（s） in A. mediterranei other than those encoded by the r/f cluster should be involved in this process.Amycolatopsis mediterranei produces an important anti- biotic rifamycin, the biosynthesis of which involves many unusual modifications. Previous work suggested a puta- tive P450 enzyme encoded by rill6 within the rifamycin biosynthetic gene cluster （rif） was required for the conver- sion of the intermediate rifamycin SV into the end product rifamycin B. In this study, we genetically proved that a putative transketolase encoded by rill5 is another essential enzyme for this conversion. Expression of merely rill5 and rifl6 in a r/f cluster null mutant ofA. mediterranei U32 was able to convert rifamycin SV into B. However, this Rifl5- and Rifl6-mediated conversion was only detected in intact cells of A. meidterranei, but not in Streptomyce coelicolor or Mycobacterium smegmatis, suggesting that yet-characterized gene（s） in A. mediterranei other than those encoded by the r/f cluster should be involved in this process.

关 键 词：Amycolatopsis mediterranei RIFAMYCIN P450 TRANSKETOLASE 

分 类 号：Q939.117[生物学—微生物学] TQ927[轻工技术与工程—发酵工程]