Identification and characterization of neuroblasts in the subventricular zone and rostral migratory stream of the adult human brain  

作　　者：Congmin Wang Fang Liu Ying-Ying Liu Cai-Hong Zhao Yan You Lei Wang Jingxiao Zhang Bin Wei Tong Ma Qiangqiang Zhang Yue Zhang Rui Chen Hongjun Song Zhengang Yang 

机构地区：[1]Institutes of Brain Science and State Key Laboratory of Medical Neurobiology,Fudan University,138 Yixueyuan Road,Shanghai 200032,China [2]Institute of Neurosciences,The Fourth Military Medical University,Xi'an 710032,China [3]Department of Human Anatomy,Hebei Medical University,Shijiazhuang 050017,China [4]Department of Obstetrics and Gynecology,The Fourth Hospital of Shijiazhuang,Shijiazhuang 050011,China [5]Institute for Cell Engineering,Department of Neurology,Johns Hopkins University School of Medicine,Baltimore,MD 21205,USA

出　　处：《Cell Research》2011年第11期1534-1550,共17页细胞研究(英文版)

基　　金：This work was supported by the National Natural Science Foundation of China(30870804,30900425,30970949,30990261,30821002 and 31028009);Shanghai Shu-Guang project(09SG05);National Basic Research Program of China(2010CB945500,2011CB504400).

摘　　要：It is of great interest to identify new neurons in the adult human brain,but the persistence of neurogenesis in the subventricular zone(SVZ)and the existence of the rostral migratory stream(RMS)-like pathway in the adult human forebrain remain highly controversial.In the present study,we have described the general configuration of the RMS in adult monkey,fetal human and adult human brains.We provide evidence that neuroblasts exist continuously in the anterior ventral SVZ and RMS of the adult human brain.The neuroblasts appear singly or in pairs without forming chains;they exhibit migratory morphologies and co-express the immature neuronal markers doublecortin,polysialylated neural cell adhesion molecule and-tubulin.Few of these neuroblasts appear to be actively proliferating in the anterior ventral SVZ but none in the RMS,indicating that neuroblasts distributed along the RMS are most likely derived from the ventral SVZ.Interestingly,no neuroblasts are found in the adult human olfactory bulb.Taken together,our data suggest that the SVZ maintains the ability to produce neuroblasts in the adult human brain.在成年人的前脑遗体在subventricular地区(SVZ)和象小径一样的喙的迁移的溪流(RMS)的存在在神经发生的成年人的大脑,而是坚持识别新神经原兴趣大高度争论。在现在的学习,我们在成年猴子,胎儿的人和成年人的大脑描述了RMS的一般配置。我们提供神经母细胞在成年人的大脑的前面的腹的SVZ和RMS连续地存在的证据。没有形成链,神经母细胞单身地或在对出现;他们展出迁移的形态学和合作快车不成熟的neuronal标记doublecortin,polysialylated神经房间粘附分子和βIII导管素。很少这些神经母细胞看起来活跃地在前面的腹的SVZ正在增殖,显示沿着RMS散布的神经母细胞是最可能的但是任何一个都没在RMS源于腹的SVZ。有趣地,没有神经母细胞在成年人的嗅觉的灯泡被发现。一起拿,我们的数据建议SVZ维持能力在成年人的大脑生产神经母细胞。

关 键 词：HUMAN rhesus monkey stem cells NEUROGENESIS NEUROBLASTS subventricular zone rostral migratory stream 

分 类 号：Q426[生物学—神经生物学] Q983.5[生物学—生理学]