果胶酶辅助提取茎瘤芥叶片中叶绿素的工艺研究  

Study on the Extraction Technology of Chlorophyll from Leaves of Brassica juncea var.tumida Tsen et Lee by Pectinase-assisted Method

作　　者：李敏[1] 

机构地区：[1]长江师范学院生命科学与技术学院,重庆涪陵408000

出　　处：《安徽农业科学》2011年第22期13380-13382,13384,共4页Journal of Anhui Agricultural Sciences

基　　金：重庆市教委科技项目(KJ101316)

摘　　要：[目的]优化果胶酶辅助有机溶剂提取茎瘤芥(Brassica juncea var.tumida Tsen et Lee)叶片中叶绿素的提取工艺。[方法]以新鲜茎瘤芥叶为原料,研究果胶酶辅助有机溶剂提取茎瘤芥叶片中叶绿素工艺,并对工艺进行优化。通过单因素和二次回归正交旋转组合设计试验,研究酶解温度、乙醇体积分数、提取时间和提取剂体积对茎瘤芥叶片叶绿素提取率的影响。[结果]茎瘤芥叶片叶绿素提取优化工艺为酶解温度10℃,乙醇体积分数40%,提取时间60 min,提取剂体积70 ml,茎瘤芥叶片叶绿素理论得率为32.29 mg/g,其实测值为31.87 mg/g,与理论值接近。[结论]该研究可为合理充分利用茎瘤芥资源,提高叶绿素提取率提供参考。[Objective] To optimize the extraction technology of chlorophyll from fresh leaves of Brassica juncea var.tumida Tsen et Lee by pectinase-assisted method.[Method] Effects of enzymolysis temperature,ethanol volume fraction,extraction time and volume of extracting solvent on the extraction of chlorophyll from fresh leaves of Brassica juncea var.tumida Tsen et Lee by pectinase-assisted method were investigated through single factor test and quadratic regression rotation-orthogonal combination design.[Result] The optimum extraction conditions were as follows： enzymolysis temperature of 10 ℃,ethanol volume fraction of 40%,extraction time of 60 min,and volume of extracting solvent of 70 ml.The theoretical extraction rate of chlorophyll was 32.29 mg/g which was approximated to the experimental value of 31.87 mg/g.[Conclusion] This study could provide reference for rational development and utilization of Brassica juncea var.tumida Tsen et Lee and improvement of extraction rate of chlorophyll.

关 键 词：茎瘤芥(Brassica JUNCEA var. tumida Tsenet Lee) 叶绿素 提取工艺 果胶酶 

分 类 号：S37[农业科学—农产品加工]